The distribution of serotonin immunoreactivity in the rat locus ceruleus after intraventricular injections of either 5,6- or 5,7-dihydroxytryptamine with special reference to serotonin synthesis

Acta histochemica
K IijimaR Kobayashi

Abstract

The localization of serotonin-immunoreactivity (5-HT-IR) in the locus ceruleus (LC) of rats was studied by the peroxidase-anti-peroxidase method using a purified antibody obtained from a rabbit. Antibody production was performed according to the method of Grota and Brown (1974). The antibody was applied to serial cryostat sections with alternate counterstaining by cresyl violet, after intraventricular injections of 5,6-dihydroxytryptamine or 5,7-dihydroxytryptamine prior to treatment with pargyline and a precursor of 5-HT. The majority of LC neurons were immunopositive, and more than half of all LC neurons clearly showed 5-HT-IR. Although core cells were the most predominant, all types of neurons were immunopositive, and randomly scattered throughout the LC. The uptake inhibitor, Lilly 110140, administered in sufficient amounts prior to an injection of pargyline, did not reduce 5-HT-IR within the LC. The results suggest that LC neurons receive 5-hydroxytryptophan (5-HTP) through an afferent vascular-neuronal channel and/or by diffusion from blood capillaries much more than 5-HT itself. We consider from these results that all types of LC neurons throughout the nucleus are masked 5-HT cells, and that the majority of LC neurons ut...Continue Reading

References

Aug 1, 1979·The American Journal of Anatomy·D L Felten, K A Crutcher
Jan 1, 1977·Progress in Neurobiology·D G Amaral, H M Sinnamon
Mar 1, 1978·The Journal of Comparative Neurology·J M Cedarbaum, G K Aghajanian
Oct 24, 1975·Brain Research·M J BrownsteinJ S Kizer
May 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, N H Sternberger
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Mar 1, 1974·Canadian Journal of Biochemistry·L J Grota, G M Brown
Jul 1, 1974·The Journal of Comparative Neurology·L C ConradD W Pfaff
Jan 1, 1974·Cell and Tissue Research·E Ramon-Moliner
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·H G Baumgarten, L Lachenmayer
Dec 21, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·A BjörklundU Stenevi
Jan 1, 1972·Zeitschrift für Zellforschung und mikroskopische Anatomie·H G BaumgartenA Nobin
Mar 1, 1970·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·N Shimizu, K Imamoto
Jun 1, 1984·Brain Research Bulletin·T SakumotoT Maeda
Jul 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E C Azmitia, W F Marovitz
Jan 1, 1980·Neuroscience·R Grzanna, M E Molliver
Jul 17, 1981·Neuroscience Letters·M FrankfurtE C Azmitia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.