The diurnal periodicity of hexose transporter mRNA and protein levels in the rat jejunum: role of vagal innervation

Surgery
Scott G HoughtonMichael G Sarr

Abstract

Protein and messenger RNA (mRNA) levels of the hexose transporters sodium-dependent glucose transporter-1, glucose transporter 2, and glucose transporter 5 follow a (daily) diurnal rhythm in rat jejunum. Because vagal innervation mediates the diurnal activity of other proteins in the rat small bowel, we hypothesized that the diurnal variation of mRNA and protein levels of these hexose transport proteins are mediated by vagal innervation. Forty-eight rats kept in a strictly maintained, alternating 12-hour light-dark room underwent either sham laparotomy (n = 24) or bilateral total abdominal vagotomy (n = 24). Four weeks postoperatively, jejunal mucosa was harvested from 6 rats in each group at 3 am, 9 am, 3 pm and 9 pm; mRNA levels were determined by reverse transcription real-time polymerase chain reaction and protein levels by semiquantitative Western blot analysis. Transporter mRNA and protein levels were expressed as a ratio to the corresponding mRNA and protein levels of the stably expressed housekeeping gene glyceraldehyde-6-phosphate dehydrogenase. mRNA and protein levels for all 3 hexose transporters showed diurnal variation in sham controls (P < or = .01 for all). After vagotomy, although mRNA levels of all 3 transporte...Continue Reading

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Citations

Nov 26, 2011·Digestive Diseases and Sciences·Ye Zheng, Michael G Sarr
Mar 19, 2011·FEBS Letters·Andries KalsbeekRuud M Buijs
Aug 23, 2011·Progress in Neurobiology·Fadi H Mourad, Nayef E Saadé
May 3, 2011·The Journal of Surgical Research·Jeffrey S ScowMichael G Sarr
Aug 27, 2010·The Journal of Surgical Research·Jeffrey S ScowMichael G Sarr
Jul 2, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jayaraman SelvarajKarundevi Balasubramanian
Aug 7, 2007·Gastroenterology·Martin SládekAlena Sumová
Apr 17, 2013·Clinical and Experimental Pharmacology & Physiology·Chooi Yeng Lee
Mar 7, 2013·Acta Physiologica·J Pácha, A Sumová
Sep 11, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Christudas SunilSavarimuthu Ignacimuthu
Dec 17, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Javairiah FatimaMichael G Sarr
Aug 27, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Hisham G QandeelMichael G Sarr
Sep 4, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·C W IqbalM G Sarr
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adam T StearnsAli Tavakkolizadeh
Nov 10, 2011·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Rizwan M ChaudhryMichael G Sarr
Aug 9, 2011·Essays in Biochemistry·Andries KalsbeekRuud M Buijs
Jan 26, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiaoqing XuWenyi Kang

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