The diurnal profile of gastric pepsin activity is reduced with Helicobacter pylori infection

Digestive Diseases and Sciences
J L NewtonA Allen

Abstract

Both Helicobacter pylori and pepsin are proven mucosal damaging agents and implicated in the aetiology of peptic ulcer disease. Historically studies of pepsin over time have proved methodologically difficult, and as a result little work has been done on the effect of H. pylori on luminal pepsin secretion. Our objectives were to determine pepsin activity over 24 hr in normal human subjects and to examine luminal pepsin activity in relation to H. pylori infection. Twenty-seven healthy volunteers had gastric juice samples aspirated every 2 hr for 24 hr. All subjects had H. pylori status determined by C13 urea breath test and serology. Meals were standardized throughout the study period. Gastric juice samples were measured for pH, diluted, and frozen in acetate buffer pH 4.1 for up to 1 month, conditions shown to cause no loss of activity. Individual samples were measured for pepsin activity by assaying for new N-terminal peptide formation. Mean pepsin activity (microg enzyme/ml) in 21 normal H. pylori-negative subjects ranged from 114 to 1030 microg/ml, with a characteristic diurnal profile of increasing activity to maximum after the evening meal. Mean pepsin activity in subjects with H. pylori was consistently below that for age-...Continue Reading

Citations

Oct 13, 2005·Current Opinion in Gastroenterology·Mitchell L Schubert
Mar 4, 2010·Archives of Oral Biology·Nadine SchlueterCarolina Ganss
May 5, 2012·Archives of Oral Biology·Nadine SchlueterCarolina Ganss
Dec 20, 2007·Acta Odontologica Scandinavica·Nadine SchlueterJoachim Klimek
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·M AlmingerC N Santos

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