The Diversified O-Superfamily in Californiconus californicus Presents a Conotoxin with Antimycobacterial Activity

Toxins
Johanna Bernáldez-SarabiaAlexei Licea-Navarro

Abstract

Californiconus californicus, previously named Conus californicus, has always been considered a unique species within cone snails, because of its molecular, toxicological and morphological singularities; including the wide range of its diet, since it is capable of preying indifferently on fish, snails, octopus, shrimps, and worms. We report here a new cysteine pattern conotoxin assigned to the O1-superfamily capable of inhibiting the growth of Mycobacterium tuberculosis (Mtb). The conotoxin was tested on a pathogen reference strain (H37Rv) and multidrug-resistant strains, having an inhibition effect on growth with a minimal inhibitory concentration (MIC) range of 3.52⁻0.22 μM, similar concentrations to drugs used in clinics. The peptide was purified from the venom using reverse phase high-performance liquid chromatography (RP-HPLC), a partial sequence was constructed by Edman degradation, completed by RACE and confirmed with venom gland transcriptome. The 32-mer peptide containing eight cysteine residues was named O1_cal29b, according to the current nomenclature for this type of molecule. Moreover, transcriptomic analysis of O-superfamily toxins present in the venom gland of the snail allowed us to assign several signal peptides...Continue Reading

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Citations

May 24, 2020·Biomedicines·David T WilsonNorelle L Daly
Feb 12, 2021·Natural Product Reports·Anthony R CarrollMichèle R Prinsep

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Methods Mentioned

BETA
amidation
PCR

Software Mentioned

HMMER
FastQC
SignalP
TrinityStats
Trinity
Basic Local Alignment Search Tool ( BLAST )
Biggs
MAFFT
fastq

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