The DMCdrive: practical 3D-printable micro-drive system for reliable chronic multi-tetrode recording and optogenetic application in freely behaving rodents.

Scientific Reports
Hoseok KimMarie Carlén

Abstract

Electrophysiological recording and optogenetic control of neuronal activity in behaving animals have been integral to the elucidation of how neurons and circuits modulate network activity in the encoding and causation of behavior. However, most current electrophysiological methods require substantial economical investments and prior expertise. Further, the inclusion of optogenetics with electrophysiological recordings in freely moving animals adds complexity to the experimental design. Expansion of the technological repertoire across laboratories, research institutes, and countries, demands open access to high-quality devices that can be built with little prior expertise from easily accessible parts of low cost. We here present an affordable, truly easy-to-assemble micro-drive for electrophysiology in combination with optogenetics in freely moving rodents. The DMCdrive is particularly suited for reliable recordings of neurons and network activities over the course of weeks, and simplify optical tagging and manipulation of neurons in the recorded brain region. The highly functional and practical drive design has been optimized for accurate tetrode movement in brain tissue, and remarkably reduced build time. We provide a complete...Continue Reading

References

Aug 20, 1993·Science·M A Wilson, B L McNaughton
Nov 22, 2001·Journal of Neuroscience Methods·M S Fee, A Leonardo
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christoph StosiekArthur Konnerth
Sep 27, 2003·Journal of Neuroscience Methods·Yannick Jeantet, Yoon H Cho
Feb 1, 2005·Neuroscience·N Schmitzer-TorbertA D Redish
Oct 21, 2005·IEEE Transactions on Bio-medical Engineering·Jit MuthuswamyAaron Gilletti
Sep 15, 2007·Journal of Neurophysiology·Andrew Jackson, Eberhard E Fetz
Apr 24, 2009·Journal of Visualized Experiments : JoVE·David P NguyenMatthew A Wilson
Apr 28, 2009·Nature·Jessica A CardinChristopher I Moore
Apr 28, 2009·Nature·Vikaas S SohalKarl Deisseroth
Jul 13, 2011·Neuron·Ofer YizharKarl Deisseroth
Dec 6, 2011·Nature Neuroscience·Polina AnikeevaKarl Deisseroth
May 19, 2012·Nature Reviews. Neuroscience·György BuzsákiChristof Koch
Nov 7, 2012·Journal of Visualized Experiments : JoVE·Kevin Ung, Benjamin R Arenkiel
Oct 8, 2013·Nature·Hyun-Jae PiAdam Kepecs
Dec 10, 2013·Neuron·Eran StarkGyörgy Buzsáki
Aug 26, 2014·Nature Neuroscience·Joshua H SiegleChristopher I Moore
Jan 16, 2016·Cell·Hoseok KimMarie Carlén
Mar 18, 2017·Nature Reviews. Neuroscience·Christina K KimKarl Deisseroth
May 29, 2018·Journal of Visualized Experiments : JoVE·Li LuDora E Angelaki
Jun 7, 2018·Frontiers in Neural Circuits·Sebastien DelcassoAnn M Graybiel
Aug 21, 2018·Neuron·Jesse JacksonAlbert K Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Software Mentioned

Makerbot
custom
MATLAB
MathWorks
SLIQ hyperdrive
MClust
Autodesk Inventor
flexDrive
systemDrive
VersaDrive

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.