PMID: 8604337Mar 15, 1996Paper

The DNA bend angle and binding affinity of an HMG box increased by the presence of short terminal arms

Nucleic Acids Research
M Lnenicek-AllenC Crane-Robinson

Abstract

The HMG box of human LEF-1 (hLEF-1, formerly TCF1alpha) has been expressed in four forms: a parent box of 81 amino acids and constructs having either a 10 amino acid C-terminal extension, a 9 amino acid N-terminal extension, or both. These four species have been compared for DNA binding and bending ability using a 28 bp recognition sequence from the TCR alpha-chain enhancer. In the bending assay, whereas the parent box and that with the N-terminal extension bent the DNA by 57/58 degrees, the box extended at the C-terminus bent the DNA by 77/78 degrees, irrespective of the presence or absence of the N-terminal extension. A 6- fold increase in DNA affinity also resulted from addition of both terminal extensions. These observations redefine the functional boundaries of the HMG box. The structure of a mouse LEF-1/DNA complex recently published [Love et al. (1995) Nature 376, 791-795] implies that the higher DNA affinity and in particular the increased bend angle observed are consequences, at least in part, of the C-terminal extension spanning the major groove on the inside of the DNA bend.

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F Diffley, B Stillman
Dec 20, 1994·Biochemistry·C S ChowS J Lippard
Jan 1, 1994·Methods in Molecular Biology·C Zwieb, S Adhya
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·K GieseR Grosschedl
Mar 1, 1994·Trends in Genetics : TIG·R GrosschedlJ Pagel
Jul 25, 1993·Nucleic Acids Research·C M ReadD G Norman
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P M PilS J Lippard

❮ Previous
Next ❯

Citations

Sep 29, 1999·Biochimica Et Biophysica Acta·J R WiśniewskiT Heyduk
Jul 27, 2001·Gene·A C SparkesC Crane-Robinson
Feb 19, 2000·Current Opinion in Structural Biology·A Travers
Mar 14, 2001·Trends in Biochemical Sciences·J O Thomas, A A Travers
Feb 1, 1997·Nature Structural Biology·C M Read, P C Driscoll
May 6, 2008·Molecular Human Reproduction·I Sánchez-MorenoP Canto
Mar 27, 2001·Protein Science : a Publication of the Protein Society·P D CaryC Crane-Robinson
Apr 12, 2006·Eukaryotic Cell·Sylvie BriquetCatherine Vaquero
Nov 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Martijn S LuijsterburgRemus Th Dame
Nov 3, 2006·Biopolymers·Micah J McCauley, Mark C Williams
Sep 29, 2004·Journal of Molecular Biology·Anatoly I DraganPeter L Privalov
Feb 20, 2003·Archives of Biochemistry and Biophysics·Qiang WuPrakash P Kumar
Apr 19, 2005·Biophysical Journal·Micah McCauleyMark C Williams
Aug 22, 2006·Trends in Biochemical Sciences·Colyn Crane-RobinsonPeter L Privalov
Jul 4, 2006·Journal of Molecular Biology·Katherine StottJean O Thomas
Mar 4, 2003·EMBO Reports·Andrew A Travers
May 9, 1998·The Journal of Biological Chemistry·M DesclozeauxP Berta
Oct 20, 2001·The Journal of Biological Chemistry·B LiM A Weiss
Mar 17, 2004·Biochimica Et Biophysica Acta·Gernot Längst, Peter B Becker
Sep 22, 2021·Journal of the American Chemical Society·Aaron T BalanaMatthew R Pratt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.