The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco

Planta
Yusuke KatoF Sato

Abstract

Plastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wild-type tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation of nitrogen during senescence. The possible regulation of protease activity of CND41 through DNA-binding is discussed.

References

Apr 18, 1994·FEBS Letters·G KoelschM Fusek
Oct 1, 1995·Trends in Biochemical Sciences·G C Walker
Jan 3, 1997·The Journal of Biological Chemistry·G K FuD M Markovitz
Apr 1, 1997·Current Opinion in Biotechnology·H G Nam
Mar 6, 1999·Current Opinion in Plant Biology·A B Bleecker
May 25, 2001·Journal of Experimental Botany·A K Tobin, T Yamaya
Mar 26, 2002·Journal of Experimental Botany·Stefan Hörtensteiner, Urs Feller
Jul 1, 1995·The Plant Cell·S. Gutteridge, A. A. Gatenby
Dec 24, 2002·Current Opinion in Plant Biology·Satoko Yoshida
Jun 24, 2003·Trends in Plant Science·Pyung Ok LimHong Gil Nam

❮ Previous
Next ❯

Citations

Dec 6, 2012·Planta·Karin KrupinskaKirsten Krause
Jun 19, 2013·Photosynthesis Research·Makoto KusabaRyouichi Tanaka
Oct 22, 2009·The Journal of Biological Chemistry·Yasuhito SakurabaAyumi Tanaka
Oct 12, 2010·Journal of Experimental Botany·Surya KantSteven J Rothstein
Sep 19, 2008·Plant Physiology·Jessica BerthelootBruno Andrieu
May 4, 2006·Annual Review of Plant Biology·Wataru Sakamoto
Oct 12, 2011·BMC Plant Biology·Juan Elías OlivaresFederico Sánchez
Nov 4, 2011·BMC Research Notes·Hatami Gigloo SedighehMasoud Ghorbani
Jan 8, 2013·Bioscience, Biotechnology, and Biochemistry·Fumihiko Sato
Mar 19, 2013·Plant Molecular Biology·Rubina JibranPaul P Dijkwel
May 3, 2014·Journal of Experimental Botany·Jean-Christophe Avice, Philippe Etienne
Apr 2, 2014·Journal of Experimental Botany·Liliana Avila-OspinaCéline Masclaux-Daubresse
Mar 7, 2014·Journal of Experimental Botany·Mercedes Díaz-MendozaIsabel Díaz
Mar 20, 2016·Plant Science : an International Journal of Experimental Plant Biology·Marine PoretJean-Christophe Avice
Dec 19, 2012·Trends in Plant Science·Jeannette Pfalz, Thomas Pfannschmidt
Dec 17, 2009·Journal of Plant Physiology·Brahim SemaneJaco Vangronsveld
Oct 10, 2007·Journal of Plant Physiology·Mira Dj MilisavljevicVesna R Maksimovic
Jun 20, 2008·The Plant Journal : for Cell and Molecular Biology·Dana E MartínezJuan J Guiamet
May 3, 2008·Plant Biotechnology Journal·Meriem BenchabaneDominique Michaud
Sep 20, 2008·Plant Biology·C Masclaux-DaubresseM Orsel
Sep 20, 2008·Plant Biology·P L GregersenK Krupinska
Mar 19, 2016·Biochimica Et Biophysica Acta·Joanna MelonekKarin Krupinska
Jan 17, 2012·Physiologia Plantarum·Irma N RobertsChristiane Funk
Apr 11, 2006·Current Opinion in Plant Biology·Zach AdamKlaas J van Wijk
Dec 21, 2010·Biochimica Et Biophysica Acta·Paul Dominic B OlinaresKlaas J van Wijk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.