PMID: 6744065Jul 9, 1984

The DNA content of cerebral cortex neurons. Determinations by cytophotometry and high performance liquid chromatography

Brain Research
R HobiC C Kuenzle

Abstract

Previous work from our laboratories has indicated that the DNA content of rat cerebral cortex neurons increases postnatally to a level of slightly above 3c, where 2c denotes the diploid DNA complement. We have re-evaluated this concept by using various cytophotometric assays and a novel high performance liquid chromatography (HPLC) technique. The latter consists of digesting the DNA in isolated neuronal nuclei by a mixture of DNA-degrading enzymes followed by analysis of the resulting deoxynucleosides by HPLC. We find that the various methods fall into two groups. The first gives evidence of a postnatal DNA (or histone) increase, while the second does not. The first group (DNA increase) comprises cytofluorometry for DNA following Schiff-type staining with fluorochromes 2,5-bis-(4-aminophenyl)-1,3,4-oxadiazole (BAO) and pararosaniline, ultraviolet absorption scanning for DNA and cytofluorometry for histones following staining with sulfaflavine at pH 8. The second group (no DNA increase) consists of cytofluorometry for DNA following staining with the DNA-complexing agents mithramycin, chromomycin A3, 4',6-diamidino-2-phenylindole (DAPI) and bisbenzimide (Hoechst 33258), as well as the newly developed HPLC technique. Since the HPL...Continue Reading

References

Jul 15, 1979·The Journal of Comparative Neurology·B De MarianisA Giuditta
Apr 1, 1978·Experimental Cell Research·C C KuenzleF Ruch
Jul 15, 1978·The Journal of Comparative Neurology·D M MannC M Barton
Jul 1, 1976·Journal of Neurochemistry·D L McIlwain, P Capps-Covey
Jan 1, 1975·Histochemistry·A BregnardC C Kuenzle
May 1, 1976·Journal of Neurobiology·R C Bohn, R B Mitchell
Jan 1, 1971·Annual Review of Biochemistry·I H Goldberg, P A Friedman
Sep 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·U Leemann, F Ruch
Jan 1, 1973·Histochemie. Histochemistry. Histochimie·S Fujita
Dec 1, 1973·The Journal of Cell Biology·A KnüselG S Kistler
Jun 1, 1970·Experimental Cell Research·V NovákováG Schlueter
May 3, 1968·Science·C J Herman, L W Lapham
Mar 1, 1969·Journal of Neurochemistry·R D Lentz, L W Lapham
Sep 1, 1969·Brain Research·C J Herman, L W Lapham
Jul 1, 1981·Cell and Tissue Kinetics·N BöhmE Kaiser
Apr 15, 1981·The Biochemical Journal·S A WhatleyL Lim
Oct 1, 1981·Experimental Cell Research·C W HeizmannC C Kuenzle

Citations

Aug 5, 1985·Neuroscience Letters·R Hobi, C C Kuenzle
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G Grassi-ZucconiA Giuditta
Oct 1, 1981·Experimental Cell Research·C W HeizmannC C Kuenzle

Related Concepts

Cell Nucleus
Cell Separation
Insula of Reil
High Pressure Liquid Chromatography Procedure
DNA, Double-Stranded
Flow Cytometry
Cavia porcellus
Neurons

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