PMID: 15228094Jul 2, 2004Paper

The DNA damaging agent VP16 induces the expression of a subset of ligands from the EGF system in bladder cancer cells, whereas none of the four EGF receptors are induced

Molecular and Cellular Biochemistry
B S SørensenE Nexø

Abstract

Increased activity of the EGF system exerts a cell survival function in the presence of cytotoxic agents. The aim of our investigation was to identify the ligands and receptors from the EGF system, that are induced by the chemotherapeutic DNA damaging agent VP16 in bladder cancer cell lines. By use of real-time RT-PCR assays for all four receptors and six ligands from the EGF system we demonstrate that in HCV29 bladder cancer cells, amphiregulin, HB-EGF, and epiregulin mRNA levels are elevated (more than 100, 5, and 4 fold, respectively) by VP16. The remaining ligands (EGF, TGFalpha and betacellulin) are uninduced. The same was found for T24A bladder cancer cells, except that TGFalpha also was induced. The four receptors were reduced by VP16 in both cell lines. This demonstrates that the induction of the EGF system is mediated by an increased expression of a subset of the ligands, whereas the four receptors are reduced. For amphiregulin and HER1 we investigated with ELISA assays if the effects of VP16 also were observed at the protein level. We found that VP16 increase the amount of amphiregulin peptide both in the cell membrane and the culture medium. Similarly, the reduced EGF receptor mRNA expression correlated with reduced ...Continue Reading

Citations

Feb 5, 2011·Genetic Testing and Molecular Biomarkers·Ronggui ZhangGuangyong Xu
Apr 9, 2009·Journal of Surgical Oncology·Eduardo SchifferCatherine M Pastor
Apr 20, 2011·Cellular Microbiology·Karen V SwansonWenxia Song
Mar 7, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zhi-Feng ZhaoHua Lu
Feb 23, 2013·The Journal of Biological Chemistry·Hironao NakayamaShigeki Higashiyama

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