The DNA repair protein NBS1 influences the base excision repair pathway

Carcinogenesis
Daniel SaganFriederike Eckardt-Schupp

Abstract

NBS1 fulfills important functions for the maintenance of genomic stability and cellular survival. Mutations in the NBS1 (Nijmegen Breakage Syndrome 1) gene are responsible for the Nijmegen breakage syndrome (NBS) in humans. The symptoms of this disease and the phenotypes of NBS1-defective cells, especially their enhanced radiosensitivity, can be explained by an impaired DNA double-strand break-induced signaling and a disturbed repair of these DNA lesions. We now provide evidence that NBS1 is also important for cellular survival after oxidative or alkylating stress where it is required for the proper initiation of base excision repair (BER). NBS1 downregulated cells show reduced activation of poly-(adenosine diphosphate-ribose)-polymerase-1 (PARP1) following genotoxic treatment with H(2)O(2) or methyl methanesulfonate, indicating impaired processing of damaged bases by BER as PARP1 activity is stimulated by the single-strand breaks intermediately generated during this repair pathway. Furthermore, extracts of these cells have a decreased capacity for the in vitro repair of a double-stranded oligonucleotide containing either uracil or 8-oxo-7,8-dihydroguanine to trigger BER. Our data presented here highlight for the first time a f...Continue Reading

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Citations

Dec 2, 2011·Mutagenesis·Agnieszka Dzikiewicz-KrawczykJerzy Nowak
Dec 31, 2009·Nucleic Acids Research·Sylvia SteiningerSimone Moertl
Dec 8, 2009·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalPremendu P Mathur
Sep 6, 2011·Mutation Research·Megumi HadaFrancis A Cucinotta

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