The DNA sequence specificity of bleomycin cleavage in a systematically altered DNA sequence

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Shweta D GautamV Murray

Abstract

Bleomycin is an anti-tumour agent that is clinically used to treat several types of cancers. Bleomycin cleaves DNA at specific DNA sequences and recent genome-wide DNA sequencing specificity data indicated that the sequence 5'-RTGT*AY (where T* is the site of bleomycin cleavage, R is G/A and Y is T/C) is preferentially cleaved by bleomycin in human cells. Based on this DNA sequence, we constructed a plasmid clone to explore this bleomycin cleavage preference. By systematic variation of single nucleotides in the 5'-RTGT*AY sequence, we were able to investigate the effect of nucleotide changes on bleomycin cleavage efficiency. We observed that the preferred consensus DNA sequence for bleomycin cleavage in the plasmid clone was 5'-YYGT*AW (where W is A/T). The most highly cleaved sequence was 5'-TCGT*AT and, in fact, the seven most highly cleaved sequences conformed to the consensus sequence 5'-YYGT*AW. A comparison with genome-wide results was also performed and while the core sequence was similar in both environments, the surrounding nucleotides were different.

References

Oct 1, 1977·Nucleic Acids Research·L F PovirkF Hutchinson
Jul 11, 1978·Biochemistry·E A SausvilleS B Horwitz
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A D D'Andrea, W A Haseltine
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M TakeshitaH Ohtsubo
Jan 9, 1979·Biochemistry·L F PovirkN Dattagupta
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J Steighner, L F Povirk
Aug 25, 1987·Nucleic Acids Research·J E GrimwadeT A Beerman
Oct 25, 1982·Journal of Molecular Biology·C R Calladine
Mar 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G StoterD Raghavan
Jun 11, 1993·Nucleic Acids Research·K P Nightingale, K R Fox
Jan 25, 1995·Nucleic Acids Research·S Belikov, L Wieslander
Jun 1, 1996·Genome Research·M InazukaK Hayashi
Jun 20, 1998·The Journal of Biological Chemistry·J W SamD L Rousseau
Oct 3, 1999·Progress in Nucleic Acid Research and Molecular Biology·V Murray
Jan 29, 2000·Journal of Natural Products·S M Hecht
Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·A Kim, V Murray
Jul 27, 2001·Journal of the American Chemical Society·S HashimotoS M Hecht
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·A R Kim, V Murray
Dec 26, 2001·Chemical Reviews·C A Claussen, E C Long
Feb 19, 2002·Chemical Reviews·Richard M. Burger
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lawrence H Einhorn
May 26, 2004·Biochimica Et Biophysica Acta·Mark D TempleVincent Murray
Dec 25, 2004·The International Journal of Biochemistry & Cell Biology·Mark D Temple, Vincent Murray
Feb 3, 2005·Nature Reviews. Cancer·Jingyang Chen, JoAnne Stubbe
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kristie D GoodwinMillie M Georgiadis
Jan 17, 2009·Journal of the American Chemical Society·Qian MaSidney M Hecht
Jun 23, 2010·Chemical Biology & Drug Design·Anne M Galea, Vincent Murray
Jul 16, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Trung V Nguyen, Vincent Murray
Mar 16, 2012·Chemical Biology & Drug Design·Vincent MurrayJon K Chen
Sep 11, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hanh T Q Nguyen, Vincent Murray
Oct 18, 2012·Journal of the American Chemical Society·Trevor C BozemanSidney M Hecht
Jan 2, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Trung V NguyenVincent Murray
Feb 6, 2013·Journal of the American Chemical Society·Zhiqiang YuSidney M Hecht

❮ Previous
Next ❯

Citations

Jan 16, 2018·Chembiochem : a European Journal of Chemical Biology·Tingting ZouHiroshi Sugiyama
May 8, 2018·International Journal of Molecular Sciences·Vincent MurrayLong H Chung

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Gene Mapper
GeneMapper

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.