The dominant negative effects of H-Ras harboring a Gly to Ala mutation at position 60.

The Journal of Biological Chemistry
Y J SungY W Hwang

Abstract

v-H-Ras harboring the Gly-60 to Ala mutation (G60A) lacks the ability to induce germinal vesicle breakdown in Xenopus oocytes. Moreover, this mutant is capable of inhibiting the activity of v-H-Ras to induce oocyte germinal vesicle breakdown when co-injected. The duration and the extent of inhibition depends on the molar ratio of v-H-Ras(G60A) to v-H-Ras. The inhibition is not due to a general toxicity of v-H-Ras(G60A) to oocytes because oocytes injected with v-H-Ras(G60A) can be readily induced to mature by other mitogenic agents, such as insulin, insulin-like growth factor 1, insulin-like growth factor 2, and phosphatidylcholine-specific phospholipase C. The dominant negative effect of v-H-Ras(G60A) requires proper membrane attachment of v-H-Ras(G60A). By using a competition assay, it was concluded that the dominant negative phenotype of v-H-Ras(G60A) resulted from sequestering H-Ras downstream effector(s). Raf-1 was identified as one of the sequestered targets.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E K ShibuyaJ V Ruderman
Dec 1, 1985·Cell·C BirchmeierM Wigler
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsI S Sigal
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1981·Advances in Protein Chemistry·J S Richardson
Apr 28, 1995·The Journal of Biological Chemistry·T R BrtvaC J Der
Jan 1, 1995·Molecular and Cellular Biology·K PumigliaR Jove
Feb 3, 1995·The Journal of Biological Chemistry·T MasudaT Kataoka
Nov 1, 1994·Trends in Biochemical Sciences·G DaumU R Rapp
Nov 1, 1994·Molecular and Cellular Biology·A KikuchiL T Williams
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·F HoferG S Martin
Aug 18, 1994·Nature·P Rodriguez-VicianaJ Downward
Jun 1, 1994·Trends in Biochemical Sciences·K J Blumer, G L Johnson
Dec 16, 1993·Nature·M S Boguski, F McCormick
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Van AelstM Wigler
Jan 1, 1993·Annual Review of Biochemistry·D R Lowy, B M Willumsen
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KoideY Kaziro

❮ Previous
Next ❯

Citations

Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·G VasudevanS M Landfear
Jan 13, 2012·The Journal of Biological Chemistry·Jan M BoitzBuddy Ullman
Mar 1, 2012·The Journal of Biological Chemistry·Zachary N WilsonPhillip A Yates
Sep 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian E HallNicolas Nassar
May 20, 2000·The Journal of Biological Chemistry·S HoffenbergB J Knoll
Aug 22, 1997·The Journal of Biological Chemistry·G J ClarkS L Campbell
May 10, 2005·The Journal of Biological Chemistry·Bradley FordNicolas Nassar
Dec 14, 2017·Biochemistry·Jia LuKenneth D Westover

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.