The domino SWI2/SNF2 Gene Product Represses Cell Death in Drosophila melanogaster

G3 : Genes - Genomes - Genetics
Kaitlyn EllisBarry Yedvobnick

Abstract

The Drosophila domino locus encodes DNA-dependent ATPases of the SWI2/SNF2 class. This class of chromatin remodeler is associated with an array of cellular activities encompassing transcription, replication, repair and recombination. Moreover, domino was observed initially to maintain a repressive chromatin state via genetic interaction studies with homeotic genes. Although domino mutations were also characterized with a cell death phenotype, its association with a death pathway has not been investigated. Here we have used targeted RNA interference to depress domino function in the wing. Resultant wing damage phenotypes were found to be enhanced through overexpression of pro-apoptotic loci, and suppressed through loss of function of these loci. Loss of wing margin and blade tissue was correlated with activation of the effector Caspase Dcp-1, a marker for apoptosis. The affected wing regions also exhibited lower levels of the DIAP1 protein, an inhibitor of apoptosis. The lower level of DIAP1 protein was not correlated with an effect on the activity of a DIAP1 gene transgenic reporter (thread-LacZ), suggesting that loss of DIAP1 occurred post transcriptionally. In some cases excessive cell proliferation within the targeted tissue...Continue Reading

References

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·A BraunM Meister
May 22, 2002·Nature Cell Biology·Hyung Don RyooHermann Steller
Jul 19, 2005·Molecular and Cellular Biology·Joel C EissenbergJohn C Chrivia
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jianrong LuPhilip Leder
Jun 6, 2009·Cell Death and Differentiation·A Bergmann
Nov 19, 2013·PLoS Genetics·Jose Rafael Morillo PradoMargaret T Fuller
Jan 10, 2014·Nature Reviews. Molecular Cell Biology·Guillermo MariñoGuido Kroemer
Mar 1, 2014·Developmental Cell·Dong YanNorbert Perrimon
Apr 1, 2017·Cell Death and Differentiation·Caitlin E Fogarty, Andreas Bergmann

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
genetic modifications

Software Mentioned

Adobe Photoshop

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.