PMID: 3760867Nov 1, 1986Paper

The dopamine metabolite 3-methoxytyramine is not a suitable indicator of dopamine release in the rat brain

Journal of Neurochemistry
A G VultoD H Versteeg

Abstract

It has been postulated that changes in the concentration of 3-methoxytyramine (3-MT) in the brain might reflect changes in the release of 3,4-dihydroxyphenylethylamine (DA, dopamine) and, therefore, might be used as an index of dopaminergic activity in the brain. 3-MT is known to accumulate rapidly after death. Killing by microwave irradiation (MWR) is considered to be the method of choice to obtain "undisturbed" 3-MT concentrations. We measured striatal 3-MT concentrations even lower than those following MWR when the brains were excised and frozen in dry ice very rapidly (typical time between decapitation and freezing of the brain 22 s). There was a linear increase in striatal 3-MT concentration when the time between decapitation and freezing was varied between 13 and 300 s. Extrapolation to time zero indicated negligible amounts of 3-MT at the time of decapitation. In addition, it was observed that DA, 3,4-dihydroxyphenylacetic acid, and homovanillic acid decompose during the cooling phase after heating the brain by microwave. It is concluded that MWR induces artifactual changes in the postmortem levels of DA and metabolites. Consequently 3-MT cannot be considered to be a reliable indicator of DA release in the rat brain.

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Citations

Jan 1, 1991·Journal of Neural Transmission. General Section·F GodefroyJ Weil-Fugazza
Jan 1, 1990·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·T BrannanM Yahr
Jan 1, 1992·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·A DingS Hoyer
Jul 19, 1991·Brain Research·S Puglisi-AllegraS Cabib
Jan 1, 1993·Physiology & Behavior·J K MiyamotoA T Miyamoto
Feb 1, 1989·Pharmacology, Biochemistry, and Behavior·W T ChanceJ E Fischer
Sep 1, 1992·Pharmacology, Biochemistry, and Behavior·A BadianiS Puglisi-Allegra
Jul 1, 1994·Progress in Neuro-psychopharmacology & Biological Psychiatry·D J HealW R Buckett
Jun 1, 1994·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·F S RadhakishunJ M van Ree
Jun 1, 1987·The Japanese Journal of Psychiatry and Neurology·K KurataN Yamaguchi
Oct 27, 2010·PloS One·Tatyana D SotnikovaRaul R Gainetdinov

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