The down-regulation of cathepsin G in THP-1 monocytes after infection with Mycobacterium tuberculosis is associated with increased intracellular survival of bacilli

Infection and Immunity
Carlos A Rivera-MarreroJesse Roman

Abstract

Cathepsin G (CatG) is a serine protease found in the azurophilic granules of monocytes that is known to have antimicrobial properties, but its role in Mycobacterium tuberculosis infection is unknown. We found that M. tuberculosis infection of human THP-1 monocytic cells induced the down-regulation of CatG mRNA expression, as demonstrated by gene array analysis and reverse transcription-PCR. This was associated with a concomitant decrease in CatG protein and enzymatic activity. In contrast, the expression of lysosomal cathepsins B and D was up-regulated in infected cells. This effect was also observed when THP-1 cells were induced to differentiate into adherent macrophages by exposure to bacterial lipopolysaccharide (LPS). In agreement with this, CatG expression was null in adherent macrophages isolated from bronchoalveolar lavages and normal blood. We wanted to determine if the down-regulation of CatG would be relevant to M. tuberculosis infection. First, we found that addition of CatG to THP-1 cells prior to infection resulted in decreased bacillary viability, presumably due to extracellular killing of bacilli. However, pretreatment of cells with LPS, which decreases intracellular CatG expression, resulted in increased bacilla...Continue Reading

References

Nov 1, 1975·The Journal of Clinical Investigation·H Odeberg, I Olsson
Dec 1, 1976·Infection and Immunity·H Odeberg, I Olsson
Aug 21, 1992·Science·B R Bloom, C J Murray
Nov 22, 1991·Journal of Immunological Methods·E M Denholm, F M Wolber
Apr 1, 1989·Inflammation·C Capodici, R A Berg
Jan 1, 1989·Molecular and Cellular Biology·T J LeyK C Robbins
Aug 1, 1989·The Journal of Clinical Investigation·R I LehrerM E Selsted
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H V WesterhoffM Zasloff
Oct 1, 1986·Infection and Immunity·W M ShaferL E Martin
Oct 1, 1971·The Journal of Experimental Medicine·D F BaintonM G Farquhar
Jan 1, 1981·Methods in Enzymology·A J Barrett
Nov 1, 1996·Infection and Immunity·P J ConverseM L Pitt
Oct 23, 2001·Respiratory Research·P J Wolters, H A Chapman
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Carlos A Rivera-MarreroJesse Roman
Feb 5, 1997·Chemical Reviews·Hans-Hartwig Otto, Tanja Schirmeister

❮ Previous
Next ❯

Citations

Aug 4, 2010·The Journal of Clinical Investigation·Stephen T ReeceStefan H E Kaufmann
Sep 24, 2014·Journal of Comparative Pathology·J P Cassidy, A R Martineau
Nov 15, 2011·Current Opinion in Microbiology·Stephen T Reece, Stefan H E Kaufmann
Aug 31, 2006·Cellular Microbiology·Andrea L Radtke, Mary X D O'Riordan
Jul 6, 2013·Acta Microbiologica Et Immunologica Hungarica·Marek FolWiesława Rudnicka
Aug 31, 2016·Scientific Reports·David PiresElsa Anes
May 22, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshiko MizushinaMasafumi Takahashi
Sep 14, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sutharsana YathursanVijayalekshmi Sarojini
Sep 17, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sabrina MüllerThomas Reinheckel
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timo BursterChristoph Driessen
Sep 7, 2018·Frontiers in Cellular and Infection Microbiology·Huynh T HopSuk Kim
Nov 26, 2019·Indian Journal of Microbiology·Srinivasan SujithaJeyaprakash Rajendhran
Mar 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kathrin SteinwedeUlrich A Maus
Jan 6, 2018·The Journal of Biological Chemistry·Huynh Tan HopSuk Kim
Nov 23, 2017·Frontiers in Immunology·Javier Arranz-TrullénEster Boix
Sep 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kerstin WalterStefan Ehlers
Feb 23, 2019·BioMed Research International·Yan Yang, Jianqing Wu
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Lidia Szulc-DąbrowskaFelix N Toka
May 1, 2021·Pharmaceuticals·Gabriel S OliveiraCátia Teixeira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.