The DpnI/DpnII pneumococcal system, defense against foreign attack without compromising genetic exchange

Mobile Genetic Elements
Calum JohnstonJean-Pierre Claverys

Abstract

Natural genetic transformation and restriction-modification (R-M) systems play potentially antagonistic roles in bacteria. R-M systems, degrading foreign DNA to protect the cell from bacteriophage, can interfere with transformation, which relies on foreign DNA to promote genetic diversity. Here we describe how the human pathogen Streptococcus pneumoniae, which is naturally transformable, yet possesses either of two R-M systems, DpnI or DpnII, accommodates these conflicting processes. In addition to the classic restrictase and double-stranded DNA methylase, the DpnII system possesses an unusual single-stranded (ss) DNA methylase, DpnA, which is specifically induced during competence for genetic transformation. We provide further insight into our recent discovery that DpnA, which protects transforming foreign ssDNA from restriction, is crucial for acquisition of pathogenicity islands.

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Citations

Sep 12, 2013·Trends in Microbiology·Calum JohnstonJean-Pierre Claverys
Dec 15, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lakshminarayan M IyerL Aravind
Feb 3, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sandra MuschiolBirgitta Henriques-Normark
Feb 11, 2014·Trends in Microbiology·Calum JohnstonJean-Pierre Claverys
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Oct 14, 2017·FEMS Microbiology Reviews·Ditte Høyer EngholmRikke Schmidt Kjærgaard
Feb 11, 2014·Nature Reviews. Microbiology·Calum JohnstonJean-Pierre Claverys
Feb 27, 2020·Microbial Genomics·Fiona Jane WhelanJames Oscar McInerney
Aug 24, 2017·FEMS Microbiology Reviews·Megan De Ste CroixMarco R Oggioni
Sep 14, 2018·Frontiers in Microbiology·Reza Rezaei JavanAngela B Brueggemann
Sep 26, 2019·Frontiers in Cellular and Infection Microbiology·Fauzy NasherLucy J Hathaway
Jan 12, 2021·Frontiers in Microbiology·Cinthia Alves-BarrocoAlexandra R Fernandes

Related Concepts

DNA
DNA Modification Methylases
DNA, Single-Stranded
Streptococcus pneumoniae
Transformation, Genetic
Endodeoxyribonuclease DpnI
Endodeoxyribonuclease DpnII
Antagonists
DpnA methylase
DNA, Double-Stranded

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