The Drosophila Circadian Clock Gates Sleep through Time-of-Day Dependent Modulation of Sleep-Promoting Neurons

Sleep
Daniel J CavanaughAmita Sehgal

Abstract

Sleep is under the control of homeostatic and circadian processes, which interact to determine sleep timing and duration, but the mechanisms through which the circadian system modulates sleep are largely unknown. We therefore used adult-specific, temporally controlled neuronal activation and inhibition to identify an interaction between the circadian clock and a novel population of sleep-promoting neurons in Drosophila. Transgenic flies expressed either dTRPA1, a neuronal activator, or Shibire(ts1), an inhibitor of synaptic release, in small subsets of neurons. Sleep, as determined by activity monitoring and video tracking, was assessed before and after temperature-induced activation or inhibition using these effector molecules. We compared the effect of these manipulations in control flies and in mutant flies that lacked components of the molecular circadian clock. Adult-specific activation or inhibition of a population of neurons that projects to the sleep-promoting dorsal Fan-Shaped Body resulted in bidirectional control over sleep. Interestingly, the magnitude of the sleep changes were time-of-day dependent. Activation of sleep-promoting neurons was maximally effective during the middle of the day and night, and was relativ...Continue Reading

References

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Citations

Apr 1, 2017·Genetics·Christine Dubowy, Amita Sehgal
Nov 7, 2017·ELife·Daniel A LeeDavid A Prober
Sep 25, 2017·Annual Review of Entomology·Charlotte Helfrich-Förster
Oct 22, 2019·Insect Science·Jie Bi, Yu-Feng Wang
Apr 19, 2020·Proceedings of the National Academy of Sciences of the United States of America·Timothy D WigginLeslie C Griffith
Sep 24, 2016·Journal of Biological Rhythms·David S GarbeAmita Sehgal
Oct 9, 2018·The European Journal of Neuroscience·Vanessa M HillMimi Shirasu-Hiza
Aug 7, 2020·Continuum : Lifelong Learning in Neurology·Logan Schneider

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