The Drosophila cytosine-5 methyltransferase Dnmt2 is associated with the nuclear matrix and can access DNA during mitosis

PloS One
Matthias SchaeferFrank Lyko

Abstract

Cytosine-5 methyltransferases of the Dnmt2 family are highly conserved in evolution and their biological function is being studied in several organisms. Although all structural DNA methyltransferase motifs are present in Dnmt2, these enzymes show a strong tRNA methyltransferase activity. In line with an enzymatic activity towards substrates other than DNA, Dnmt2 has been described to localize to the cytoplasm. Using molecular and biochemical approaches we show here that Dnmt2 is both a cytoplasmic and a nuclear protein. Sub-cellular fractionation shows that a significant amount of Dnmt2 is bound to the nuclear matrix. Sub-cellular localization analysis reveals that Dnmt2 proteins are enriched in actively dividing cells. Dnmt2 localization is highly dynamic during the cell cycle. Using live imaging we observed that Dnmt2-EGFP enters prophase nuclei and shows a spindle-like localization pattern during mitotic divisions. Additional experiments suggest that this localization is microtubule dependent and that Dnmt2 can access DNA during mitotic cell divisions. Our results represent the first comprehensive characterization of Dnmt2 proteins on the cellular level and have important implications for our understanding of the molecular a...Continue Reading

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Citations

Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guangmei ZhangSassan Asgari
Dec 17, 2009·Nucleic Acids Research·Yuri MotorinMark Helm
Oct 13, 2009·Yi chuan = Hereditas·Zhi-Gang Wang, Jian-Xin Wu
Sep 5, 2009·Chromosoma·Matthias Schaefer, Frank Lyko
Feb 2, 2013·EMBO Reports·Zeljko DurdevicMatthias Schaefer
May 18, 2013·Wiley Interdisciplinary Reviews. RNA·Tennille SibbrittThomas Preiss
Sep 11, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Zeljko Durdevic, Matthias Schaefer
May 28, 2016·RNA Biology·Albert JeltschMark Helm
Sep 27, 2018·The Journal of Experimental Biology·Varada AbhyankarGirish S Ratnaparkhi
Oct 30, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Aleksej Drino, Matthias R Schaefer
Jun 16, 2017·PLoS Pathogens·Tamanash BhattacharyaRichard W Hardy
Sep 17, 2019·Biochemistry. Biokhimii︠a︡·S A KuznetsovaO A Dontsova

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immunoprecipitation

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