PMID: 1913820Oct 4, 1991

The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily

Cell
M R KoelleD S Hogness

Abstract

The steroid hormone ecdysone triggers coordinate changes in Drosophila tissue development that result in metamorphosis. To advance our understanding of the genetic regulatory hierarchies controlling this tissue response, we have isolated and characterized a gene, EcR, for a new steroid receptor homolog and have shown that it encodes an ecdysone receptor. First, EcR protein binds active ecdysteroids and is antigenically indistinguishable from the ecdysone-binding protein previously observed in extracts of Drosophila cell lines and tissues. Second, EcR protein binds DNA with high specificity at ecdysone response elements. Third, ecdysone-responsive cultured cells express EcR, whereas ecdysone-resistant cells derived from them are deficient in EcR. Expression of EcR in such resistant cells by transfection restores their ability to respond to the hormone. As expected, EcR is nuclear and found in all ecdysone target tissues examined. Furthermore, the EcR gene is expressed at each developmental stage marked by a pulse of ecdysone.

References

May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Bangs, K White
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·G JonesV Chhokar
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·E H Baehrecke
Jan 1, 1997·Archives of Insect Biochemistry and Physiology·S R PalliA Retnakaran
Jan 1, 1997·Archives of Insect Biochemistry and Physiology·T S Dhadialla, G Tzertzinis
Jan 1, 1997·Archives of Insect Biochemistry and Physiology·C ElkeM Lezzi
Jan 25, 2000·Archives of Insect Biochemistry and Physiology·G Jayachandran, A M Fallon
Oct 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Bownes
Jan 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Lehmann
Jan 1, 1993·Developmental Genetics·V C HenrichL I Gilbert
Jan 1, 1994·Developmental Genetics·V VenkataramanG Jones
Nov 1, 1992·Journal of Neurobiology·J W TrumanS Robinow
Oct 23, 1998·Invertebrate Neuroscience : in·L I GilbertR Rybczynski
Aug 19, 2007·Development Genes and Evolution·Michie Nemoto, Kenji Hara
Dec 4, 2012·Apoptosis : an International Journal on Programmed Cell Death·Chun-Yan LiuXiao-Fan Zhao
Jun 6, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Gerald M LiewSusan A Gerbi
Jun 1, 1992·Trends in Pharmacological Sciences·L PoellingerJ A Gustafsson
Nov 16, 1993·Biochimica Et Biophysica Acta·M MisrahiE Milgrom
Mar 1, 1996·Trends in Genetics : TIG·W A Müller
Aug 30, 1995·Molecular and Cellular Endocrinology·P P D'AvinoM Furia
Dec 23, 1993·Biochimica Et Biophysica Acta·R Janknecht, A Nordheim
Feb 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M KamimuraH Fujiwara
Jan 1, 1993·Mechanisms of Development·C K Ohno, M Petkovich

Citations

Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Best-BelpommeA Rambach
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P MaroyJ D O'Connor
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M A YundJ W Fristrom
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 11, 1990·Nucleic Acids Research·J GalceránM Izquierdo
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D J MangelsdorfM R Haussler
May 1, 1989·Molecular and Cellular Endocrinology·A M Handler, P Maroy
Dec 17, 1987·Nature·V GiguereR M Evans
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A R BakerB W O'Malley
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Dec 18, 1986·Nature·C WeinbergerR M Evans

Related Concepts

Transfection
Genes, Reiterated
Proteins, Recombinant DNA
Ecdysone receptor B1
Receptors, Steroid
ecd
Homologous Sequences, Nucleic Acid
Ecdysone
Drosophila melanogaster
Restriction Mapping

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.