The Drosophila Helicase Maleless (MLE) is Implicated in Functions Distinct From its Role in Dosage Compensation.

Molecular & Cellular Proteomics : MCP
Simona CugusiJohn C Lucchesi

Abstract

Helicases are ubiquitous enzymes that unwind or remodel single or double-stranded nucleic acids, and that participate in a vast array of metabolic pathways. The ATP-dependent DEXH-box RNA/DNA helicase MLE was first identified as a core member of the chromatin remodeling MSL complex, responsible for dosage compensation in Drosophila males. Although this complex does not assemble in females, MLE is present. Given the multiplicity of functions attributed to its mammalian ortholog RNA helicase A, we have carried out an analysis for the purpose of determining whether MLE displays the same diversity. We have identified a number of different proteins that associate with MLE, implicating its role in specific pathways. We have documented this association in selected examples that include the spliceosome complex, heterogeneous Nuclear Ribonucleoproteins involved in RNA Processing and in Heterochromatin Protein 1 deposition, and the NuRD complex.

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Citations

Jan 12, 2016·PLoS Genetics·Simona CugusiJohn C Lucchesi
Mar 5, 2020·Doklady. Biochemistry and Biophysics·J V NikolenkoA N Krasnov
Jun 9, 2020·Nucleic Acids Research·Marisa MüllerPeter B Becker
Aug 17, 2018·G3 : Genes - Genomes - Genetics·Evan LongJoshua A Udall
Dec 21, 2016·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jennifer A UrbanErica N Larschan
Sep 9, 2018·Nature Communications·Claudia Isabelle Keller ValsecchiAsifa Akhtar

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Methods Mentioned

BETA
co-immunoprecipitation
Transfection
ChIP-chip
immunoprecipitations
reverse transcription-PCR
immunoprecipitation
co-IP

Software Mentioned

msn
filter mods
_ Zipper
Python
modENCODE
results
MudPIT
SEQUEST
Galaxy
extract

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