Sep 26, 2014

The Drosophila MAPK p38c regulates oxidative stress and lipid homeostasis in the intestine

PLoS Genetics
Sveta ChakrabartiBruno Lemaitre

Abstract

The p38 mitogen-activated protein (MAP) kinase signaling cassette has been implicated in stress and immunity in evolutionarily diverse species. In response to a wide variety of physical, chemical and biological stresses p38 kinases phosphorylate various substrates, transcription factors of the ATF family and other protein kinases, regulating cellular adaptation to stress. The Drosophila genome encodes three p38 kinases named p38a, p38b and p38c. In this study, we have analyzed the role of p38c in the Drosophila intestine. The p38c gene is expressed in the midgut and upregulated upon intestinal infection. We showed that p38c mutant flies are more resistant to infection with the lethal pathogen Pseudomonas entomophila but are more susceptible to the non-pathogenic bacterium Erwinia carotovora 15. This phenotype was linked to a lower production of Reactive Oxygen Species (ROS) in the gut of p38c mutants, whereby the transcription of the ROS-producing enzyme Duox is reduced in p38c mutant flies. Our genetic analysis shows that p38c functions in a pathway with Mekk1 and Mkk3 to induce the phosphorylation of Atf-2, a transcription factor that controls Duox expression. Interestingly, p38c deficient flies accumulate lipids in the intes...Continue Reading

Mentioned in this Paper

Calcinus elegans
Malpighian Tubules
Metabolic Process, Cellular
Cyartonema elegans
Real-Time Polymerase Chain Reaction
Activating Transcription Factors
ATF3 gene
Coleonyx elegans
Immune Response
Pathogenic Aspects

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