The Drosophila melanogaster DmCK2beta transcription unit encodes for functionally non-redundant protein isoforms

Gene
Eike JauchThomas Raabe

Abstract

Genes encoding for the two evolutionary highly conserved subunits of a heterotetrameric protein kinase CK2 holoenzyme are present in all examined eukaryotic genomes. Depending on the organism, multiple transcription units encoding for a catalytically active CK2alpha subunit and/or a regulatory CK2beta subunit may exist. The phosphotransferase activity of members of the protein kinase CK2alpha family is thought to be independent of second messengers but is modulated by interaction with CK2beta-like proteins. In the genome of Drosophila melanogaster, one gene encoding for a CK2alpha subunit and three genes encoding for CK2beta-like proteins are present. The X-linked DmCK2beta transcription unit encodes for several CK2beta protein isoforms due to alternative splicing of its primary transcript. We addressed the question whether CK2beta-like proteins are redundant in function. Our in vivo experiments show that variations of the very C-terminal tail of CK2beta isoforms encoded by the X-linked DmCK2beta transcription unit influence their functional properties. In addition, we find that CK2beta-like proteins encoded by the autosomal D. melanogaster genes CK2betates and CK2beta' cannot fully substitute for a loss of CK2beta isoforms enc...Continue Reading

References

Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·A I KalmykovaV A Gvozdev
Mar 8, 1999·Molecular Cell Biology Research Communications : MCBRC·A P BidwaiC V Glover
Mar 24, 2000·Science·G M RubinD A Harvey
Aug 31, 2000·Molecular Cell Biology Research Communications : MCBRC·A P BidwaiC V Glover
Nov 26, 2002·Nature·Jui-Ming LinRavi Allada
Feb 4, 2003·Nature Neuroscience·Bikem AktenF Rob Jackson
Dec 13, 2005·Molecular and Cellular Biochemistry·Karsten Niefind, Olaf-Georg Issinger
Dec 13, 2005·Molecular and Cellular Biochemistry·Olivier BlondBrigitte Boldyreff

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Citations

Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bikem AktenF Rob Jackson
Jun 19, 2009·Genesis : the Journal of Genetics and Development·Ezgi Kunttas-TatliAshok P Bidwai
Aug 30, 2008·Evolution; International Journal of Organic Evolution·Maurizio TomaiuoloDavid Houle
Jan 26, 2011·Cellular Signalling·Felix StarkThomas Raabe
Aug 26, 2006·Mechanisms of Development·Anasua BoseAshok P Bidwai
Oct 2, 2013·Developmental Biology·Yogesh P WairkarPhillip Cho
Jan 6, 2009·Biochemical and Biophysical Research Communications·Benjamin MentzelThomas Raabe

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