The Drosophila Nbs protein functions in multiple pathways for the maintenance of genome stability.

Genetics
Laura CiapponiMaurizio Gatti

Abstract

The Mre11/Rad50/Nbs (MRN) complex and the two protein kinases ATM and ATR play critical roles in the response to DNA damage and telomere maintenance in mammalian systems. It has been previously shown that mutations in the Drosophila mre11 and rad50 genes cause both telomere fusion and chromosome breakage. Here, we have analyzed the role of the Drosophila nbs gene in telomere protection and the maintenance of chromosome integrity. Larval brain cells of nbs mutants display telomeric associations (TAs) but the frequency of these TAs is lower than in either mre11 or rad50 mutants. Consistently, Rad50 accumulates in the nuclei of wild-type cells but not in those of nbs cells, indicating that Nbs mediates transport of the Mre11/Rad50 complex in the nucleus. Moreover, epistasis analysis revealed that rad50 nbs, tefu (ATM) nbs, and mei-41 (ATR) nbs double mutants have significantly higher frequencies of TAs than either of the corresponding single mutants. This suggests that Nbs and the Mre11/Rad50 complex play partially independent roles in telomere protection and that Nbs functions in both ATR- and ATM-controlled telomere protection pathways. In contrast, analysis of chromosome breakage indicated that the three components of the MRN c...Continue Reading

References

Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M GattiB S Baker
Dec 9, 1998·Molecular Cell·L FantiS Pimpinelli
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·G LuoJ H Petrini
Jul 13, 2000·American Journal of Human Genetics·J GoodshipM Wright
Feb 19, 2002·Oncogene·Titia de Lange
Feb 19, 2002·Oncogene·Tej K Pandita
May 4, 2002·Nature Reviews. Molecular Cell Biology·Damien D'Amours, Stephen P Jackson
Jan 3, 2003·Nature Cell Biology·Giovanni CenciMaurizio Gatti
Mar 4, 2003·Nature Reviews. Cancer·Yosef Shiloh
Oct 9, 2003·The EMBO Journal·Tamar UzielYosef Shiloh
Jul 6, 2004·The Journal of Biological Chemistry·Karen Cerosaletti, Patrick Concannon
Jul 20, 2004·Chromosoma·Kenta IijimaHiroshi Tauchi
Aug 4, 2004·Genes & Development·Fabrizio d'Adda di FagagnaStephen P Jackson
Aug 7, 2004·Current Biology : CB·Elizabeth SilvaShelagh D Campbell
Aug 17, 2004·Chromosoma·Nora Assenmacher, Karl-Peter Hopfner
Dec 24, 2004·The EMBO Journal·Tom StiffPenny A Jeggo
Jun 21, 2005·Nature Cell Biology·Simone DifilippantonioAndré Nussenzweig
Jul 14, 2005·Chromosoma·Giovanni CenciMaurizio Gatti
Aug 10, 2005·Human Molecular Genetics·Hui ZhongDavid O Ferguson
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiaolin BiYikang S Rong
Feb 16, 2006·Molecular and Cellular Biology·Karen CerosalettiPatrick Concannon

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Citations

Feb 5, 2008·Nature Genetics·Mariarosaria MusaròGiovanni Cenci
Feb 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Grazia D RaffaMaurizio Gatti
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Guanjun GaoYikang S Rong
Jan 12, 2011·Journal of Experimental Botany·Bernd Edlinger, Peter Schlögelhofer
Apr 12, 2007·Genome Génome / Conseil National De Recherches Canada·E A SilvaS D Campbell
Oct 22, 2010·Genome Génome / Conseil National De Recherches Canada·M PedersenS D Campbell
May 9, 2009·PLoS Genetics·Raghuvar Dronamraju, James M Mason
Sep 18, 2013·Cellular and Molecular Life Sciences : CMLS·Nick FulcherKarel Riha
Apr 28, 2009·DNA Repair·Sushmita MukherjeeJeff Sekelsky
Jul 25, 2009·The EMBO Journal·Stefan Schoeftner, Maria A Blasco
Mar 28, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rachael J Rutkowski, William D Warren
Dec 18, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·James M MasonHarald Biessmann
Nov 12, 2016·Molecular Biology and Evolution·Yuh Chwen G LeeMia T Levine
Sep 25, 2017·Genetics·Rebeccah L KurzhalsKent G Golic
Jan 21, 2016·Fly·Marta Marzullo, Maurizio Gatti
Jan 7, 2019·Chromosoma·Chiara MeriglianoFiammetta Vernì
Apr 15, 2009·Genetics·Laura M McNamee, Michael H Brodsky
Dec 15, 2019·Cell Death & Disease·Giuseppe BossoGiovanni Cenci
Feb 9, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kinyo OnYasuko Kato
Apr 25, 2015·Journal of Cell Science·Raphaëlle Dubruille, Benjamin Loppin

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