PMID: 7523244Mar 1, 1994Paper

The Drosophila orb RNA-binding protein is required for the formation of the egg chamber and establishment of polarity

Genes & Development
V LantzP Schedl

Abstract

The orb gene of Drosophila encodes sex-specific germ-line proteins that contain two RRM-type RNA-binding domains. Here we report the distribution of Orb protein in wild-type, tumorous, and orb mutant ovaries. The wild-type distribution of Orb protein during oogenesis resembles that of its RNA, preferentially accumulating in the cytoplasm of the developing oocyte shortly after the formation of the 16-cell cyst. As anticipated from its germ-line expression, mutations in orb lead to female sterility. Analysis of the effect of orb mutants on the distribution of RNAs known to be required for oocyte differentiation and polarity suggests that orb functions in RNA localization at multiple points during oogenesis. In addition, phenotypic characterization of the orb mutants indicates that the gene is required early in oogenesis for formation of the 16-cell cyst. It then functions in the differentiation of the oocyte and is required for the three-dimensional reorganization of the germ cells in the cyst as well as for the establishment of normal germ-line-soma interactions in the egg chamber.

Citations

Jan 1, 1996·Archives of Insect Biochemistry and Physiology·M M Morgan, A P Mahowald
Mar 1, 1996·Trends in Genetics : TIG·C Rongo, R Lehmann
Nov 16, 2005·Comptes rendus biologies·Federica PiccioniArturo C Verrotti
Jul 30, 2003·Developmental Biology·Nicholas S Sokol, Lynn Cooley
Oct 1, 1996·Current Opinion in Genetics & Development·G Seydoux
Jun 17, 1999·Trends in Cell Biology·M E PeplingA C Spradling
Jan 10, 2002·RNA·N MinshallN Standart
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·R Mendez, J D Richter
Mar 1, 2013·Nature Reviews. Cancer·Andrea D'AmbrogioJoel D Richter
Dec 1, 1996·Differentiation; Research in Biological Diversity·K A HessJ P Petschek
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A C VerrottiM Wickens
Oct 21, 2000·Genes & Development·C LuitjensM Wickens
Dec 4, 2001·Genes & Development·S L Page, R S Hawley
Oct 6, 1998·Annual Review of Biochemistry·A BashirullahH D Lipshitz
Jan 19, 1999·Annual Review of Cell and Developmental Biology·N K Gray, M Wickens
Jan 1, 1997·Annual Review of Genetics·M de CuevasA C Spradling
Sep 23, 2009·PLoS Biology·Ervin L JohnsonGraeme W Davis
Dec 14, 2011·PloS One·Li Chin Wong, Paul Schedl
Sep 27, 2013·PloS One·Alexandre CostaPaul Schedl

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.