The Drosophila SUN protein Spag4 cooperates with the coiled-coil protein Yuri Gagarin to maintain association of the basal body and spermatid nucleus.

Journal of Cell Science
Martin P KracklauerKathleen M Beckingham

Abstract

Maintaining the proximity of centrosomes to nuclei is important in several cellular contexts, and LINC complexes formed by SUN and KASH proteins are crucial in this process. Here, we characterize the presumed Drosophila ortholog of the mammalian SUN protein, sperm-associated antigen 4 (Spag4, previously named Giacomo), and demonstrate that Spag4 is required for centriole and nuclear attachment during spermatogenesis. Production of spag4 mRNA is limited to the testis, and Spag4 protein shows a dynamic pattern of association with the germline nuclei, including a concentration of protein at the site of attachment of the single spermatid centriole. In the absence of Spag4, nuclei and centrioles or basal bodies (BBs) dissociate from each other after meiosis. This role of Spag4 in centriolar attachment does not involve either of the two KASH proteins of the Drosophila genome (Klarsicht and MSP-300), but does require the coiled-coil protein Yuri Gagarin. Yuri shows an identical pattern of localization at the nuclear surface to Spag4 during spermatogenesis, and epistasis studies show that the activities of Yuri and dynein-dynactin are downstream of spag4 in this centriole attachment pathway. The later defects in spermatogenesis seen fo...Continue Reading

References

Jan 1, 1975·Journal of Ultrastructure Research·K T Tokuyasu
Aug 1, 1974·Journal of Ultrastructure Research·K T Tokuyasu
Dec 10, 1999·Molecular Biology of the Cell·J L HicksM Bownes
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·M DregerF Hucho
Aug 10, 2002·Science·Daniel A Starr, Min Han
Feb 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wei J Gong, Kent G Golic
Nov 14, 2003·Cell and Tissue Research·Claire KennedyMoira K O'Bryan
Apr 15, 2004·The Journal of Biological Chemistry·Didier M HodzicPhilip D Stahl
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Yosef GruenbaumKatherine L Wilson
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Daniel A Starr, Janice A Fischer
Dec 7, 2005·The Journal of Cell Biology·Kevin WilhelmsenArnoud Sonnenberg
Dec 29, 2005·The Journal of Cell Biology·Melissa CrispDidier Hodzic
Apr 6, 2006·Genes, Brain, and Behavior·J D ArmstrongK M Beckingham
Aug 29, 2007·The Journal of Cell Biology·Qian LiuKyle J Roux
Sep 21, 2007·Journal of Cell Science·Mirjam KetemaArnoud Sonnenberg
Apr 9, 2008·Experimental Cell Research·P J Stewart-HutchinsonDidier Hodzic
May 15, 2008·Journal of Cell Science·Michael J TexadaKathleen M Beckingham
May 15, 2008·Journal of Cell Science·Yvonne LükeAngelika A Noegel
Oct 25, 2008·Nucleic Acids Research·Susan TweedieUNKNOWN FlyBase Consortium
Apr 10, 2009·Molecular Biology of the Cell·Michael A AndersonLaura A Lee

❮ Previous
Next ❯

Citations

Jun 30, 2011·DNA and Cell Biology·Xian-Zhen JiangXiao-Wei Xing
Sep 10, 2011·Skeletal Muscle·James Dr Knight, Rashmi Kothary
May 17, 2011·Vascular Cell·Peter C RaeJustin C St John
Mar 24, 2011·Development·Caroline V SartainMariana F Wolfner
Jan 30, 2014·PloS One·Katherine TaylorDeborah A Kimbrell
Oct 5, 2014·Journal of Molecular Biology·Nisha M Ramdas, G V Shivashankar
Sep 30, 2015·Developmental Biology·Alessandra CalviBrian Burke
Oct 13, 2015·Nature Cell Biology·Nicolas ChristophorouJean-René Huynh
Jun 28, 2013·Biology of the Cell·Daniel S Osorio, Edgar R Gomes
Jan 16, 2013·Cell Biology International·Anton V Burakov, Elena S Nadezhdina
Aug 8, 2015·Open Biology·Benjamin LoppinBéatrice Horard
May 18, 2013·Nucleus·Xiao Zhou, Iris Meier
Oct 23, 2012·Spermatogenesis·Lacramioara Fabian, Julie A Brill
Mar 6, 2015·Journal of Experimental Botany·Xiao ZhouIris Meier
Oct 13, 2017·Biology of Reproduction·Mari S Lehti, Anu Sironen
Jan 3, 2019·Molecular Biology of the Cell·Céline AugièreBénédicte Durand
Feb 27, 2015·Biological Chemistry·Dae In KimKyle J Roux
Mar 15, 2019·International Journal of Molecular Sciences·Chung-Hsin YehYing-Hung Lin
Sep 5, 2019·Archives of Insect Biochemistry and Physiology·John C BiwotYu-Feng Wang
Dec 18, 2020·Cells·Maria Giovanna RiparbelliGiuliano Callaini
Mar 5, 2021·Archives of Insect Biochemistry and Physiology·Meng-Yan ChenYu-Feng Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.