The Drosophila TRPP cation channel, PKD2 and Dmel/Ced-12 act in genetically distinct pathways during apoptotic cell clearance.

PloS One
Emeline Van GoethemNathalie C Franc

Abstract

Apoptosis, a genetically programmed cell death, allows for homeostasis and tissue remodelling during development of all multi-cellular organisms. Phagocytes swiftly recognize, engulf and digest apoptotic cells. Yet, to date the molecular mechanisms underlying this phagocytic process are still poorly understood. To delineate the molecular mechanisms of apoptotic cell clearance in Drosophila, we have carried out a deficiency screen and have identified three overlapping phagocytosis-defective mutants, which all delete the fly homologue of the ced-12 gene, known as Dmel\ced12. As anticipated, we have found that Dmel\ced-12 is required for apoptotic cell clearance, as for its C. elegans and mammalian homologues, ced-12 and elmo, respectively. However, the loss of Dmel\ced-12 did not solely account for the phenotypes of all three deficiencies, as zygotic mutations and germ line clones of Dmel\ced-12 exhibited weaker phenotypes. Using a nearby genetically interacting deficiency, we have found that the polycystic kidney disease 2 gene, pkd2, which encodes a member of the TRPP channel family, is also required for phagocytosis of apoptotic cells, thereby demonstrating a novel role for PKD2 in this process. We have also observed genetic i...Continue Reading

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Citations

Dec 24, 2013·Genes & Development·Ozge E Tasdemir-Yilmaz, Marc R Freeman
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Allison K TimmonsKimberly McCall
Jan 13, 2018·Frontiers in Immunology·Qian ZhengHui Xiao
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Methods Mentioned

BETA
GTPase
confocal microscopy
dissection
transgenic
PCRs
PCR
restriction digests
electrophoresis

Software Mentioned

lasersharp
Adobe Photoshop
Image J

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