The dual-basin landscape in GFP folding.

Proceedings of the National Academy of Sciences of the United States of America
Benjamin T AndrewsPatricia A Jennings

Abstract

Recent experimental studies suggest that the mature GFP has an unconventional landscape composed of an early folding event with a typical funneled landscape, followed by a very slow search and rearrangement step into the locked, active chromophore-containing structure. As we have shown previously, the substantial difference in time scales is what generates the observed hysteresis in thermodynamic folding. The interconversion between locked and the soft folding structures at intermediate denaturant concentrations is so slow that it is not observed under the typical experimental observation time. Simulations of a coarse-grained model were used to describe the fast folding event as well as identify native-like intermediates on energy landscapes enroute to the fluorescent native fold. Interestingly, these simulations reveal structural features of the slow dynamic transition to chromophore activation. Experimental evidence presented here shows that the trapped, native-like intermediate has structural heterogeneity in residues previously linked to chromophore formation. We propose that the final step of GFP folding is a "locking" mechanism leading to chromophore formation and high stability. The combination of previous experimental w...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P E LeopoldJ N Onuchic
Jan 1, 1983·Annual Review of Biophysics and Bioengineering·N Go
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Nov 22, 1996·Science·P DemantM Svobodova
Jun 3, 1997·Biochemistry·B G Reid, G C Flynn
Jan 1, 1997·Annual Review of Physical Chemistry·J N OnuchicP G Wolynes
Jun 27, 1998·Journal of Molecular Biology·U ReimerG Fischer
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M SalzmannK Wüthrich
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·W WeberP W Langhoff
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·C ClementiJ N Onuchic
Sep 29, 2000·Biochemistry·H FukudaK Kuwajima
Aug 10, 2001·Journal of Biomolecular NMR·A EletskyK Pervushin
Jul 4, 2002·Journal of the American Chemical Society·Markus H J SeifertTad A Holak
Mar 26, 2003·Journal of Biomolecular NMR·Julia GeorgescuTad A Holak
Jun 6, 2003·Journal of Molecular Biology·Jan KubelkaJames Hofrichter
Oct 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·David P BarondeauElizabeth D Getzoff
Nov 3, 2004·Biochemistry·Sawako EnokiKunihiro Kuwajima
Nov 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hendrik Dietz, Matthias Rief
Apr 19, 2005·Biophysical Journal·John M Finke, José N Onuchic
Jun 23, 2005·Journal of the American Chemical Society·Satoshi HabuchiJohan Hofkens
Dec 22, 2005·Nature Biotechnology·Jean-Denis PédelacqGeoffrey S Waldo
Feb 14, 2006·Journal of Molecular Biology·Shachi GosaviJosé N Onuchic
Apr 15, 2006·Photochemistry and Photobiology·Scott L Maddalo, Marc Zimmer
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hendrik DietzMatthias Rief
May 22, 2007·Journal of Molecular Biology·Jie-rong HuangSophie E Jackson
Sep 8, 2007·Journal of Molecular Biology·Benjamin T AndrewsPatricia A Jennings
Dec 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Moritz MicklerMatthias Rief
Jan 29, 2008·Nature Structural & Molecular Biology·Andreas MartinRobert T Sauer
Feb 5, 2008·Current Opinion in Structural Biology·R Dustin SchaefferValerie Daggett
May 30, 2008·Journal of the American Chemical Society·Angel OrteDavid Klenerman
Jun 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hideaki MizunoAtsushi Miyawaki
Jul 18, 2007·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Beata Wielgus KutrowskaPatricia L Clark

❮ Previous
Next ❯

Citations

Nov 10, 1994·Biochimica Et Biophysica Acta·L MedinaA Pardo
Jul 4, 2013·Journal of the American Chemical Society·Keunbong Do, Steven G Boxer
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Govardhan ReddyD Thirumalai
Nov 11, 2009·The Journal of Biological Chemistry·Shang-Te Danny HsuSophie E Jackson
Jul 14, 2012·Reports on Progress in Physics·Paul C WhitfordJosé N Onuchic
Mar 3, 2010·Annual Review of Biophysics·Kim SneppenSzabolcs Semsey
Oct 3, 2012·PloS One·Ellinor HaglundJosé N Onuchic
Nov 13, 2012·PloS One·Olesya V StepanenkoKonstantin K Turoverov
Jun 20, 2014·PLoS Computational Biology·Ellinor HaglundPatricia A Jennings
Dec 24, 2011·The Journal of Chemical Physics·Prem P ChapagainWilliam Castillo
Jan 30, 2013·Biochimica Et Biophysica Acta·Barbara CampaniniStefano Bettati
Mar 31, 2015·International Journal of Molecular Sciences·Jovaun JacksonPaul Charles Whitford
Dec 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Géraldine KleinJean Philippe Renault
May 3, 2011·Biophysical Chemistry·Chiara BosisioMaddalena Collini
Jul 7, 2009·Journal of Molecular Biology·Benjamin T AndrewsPatricia A Jennings
Mar 10, 2016·Biophysical Journal·Dominique T Capraro, Patricia A Jennings
Apr 5, 2013·Proteins·Sandipan MohantyOlav Zimmermann
Sep 13, 2011·Protein Science : a Publication of the Protein Society·Yao-Ming HuangChristopher Bystroff
Jan 11, 2014·Protein Science : a Publication of the Protein Society·David J RosenmanChristopher Bystroff
Aug 25, 2016·Nature Chemistry·Bridget SalnaPaul M Champion
Nov 2, 2016·International Journal of Molecular Sciences·Olesya V StepanenkoKonstantin K Turoverov
Jan 12, 2017·Biochemistry·Shounak BanerjeeChristopher Bystroff
Jul 11, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Kirsten JacksonZ Hugh Fan
Sep 23, 2009·Chemical Society Reviews·Shang-Te Danny HsuSophie E Jackson
Aug 21, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·David J BurbanPatricia A Jennings
Aug 2, 2014·Acta Crystallographica. Section D, Biological Crystallography·James A J ArpinoD Dafydd Jones
Sep 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M CaraglioA Pelizzola
Dec 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Katrin WolffMarkus Porto
Mar 26, 2013·The Journal of Physical Chemistry Letters·Benjamin T AndrewsPatricia A Jennings
Mar 28, 2019·The Journal of Physical Chemistry. B·Aswathy N MuttathukattilGovardhan Reddy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Molecular Biology
Benjamin T AndrewsPatricia A Jennings
Journal of Molecular Biology
Benjamin T AndrewsPatricia A Jennings
© 2022 Meta ULC. All rights reserved