The dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses

Journal of Experimental Botany
Ainelén PiazzaNatalia Gottig

Abstract

Xanthomonas citri subsp. citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp. The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis. Expression of genes encoding trehalose-6-phosphate synthase (otsA) and trehalose phosphatase (otsB) was highly induced during canker development, suggesting that the two-step pathway of trehalose biosynthesis via trehalose-6-phosphate has a function in pathogenesis. This pathway was eliminated from the bacterium by deletion of the otsA gene. The resulting XccΔotsA mutant produced less trehalose than the wild-type strain, was less resistant to salt and oxidative stresses, and was less able to colonize plant tissues. Gene expression and proteomic analyses of infected leaves showed that infection with XccΔotsA triggered only weak defence responses in the plant compared with infection with Xcc, and had less impact on the host plant's metabolism than the wild-type strain. These results suggested that trehalose of bacterial origin, synthesized via the otsA-otsB pathway, in Xcc, plays a role in modifying the host plant's metabolism to its own advantage but is also perceived by the plant as a sign of pathogen attack. Thus,...Continue Reading

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Citations

Sep 10, 2016·PloS One·André Vessoni AlexandrinoMaria Teresa Marques Novo-Mansur
Mar 16, 2017·Journal of Bacteriology·Gabriela L MüllerMaría Alejandra Mussi
Sep 17, 2018·The New Phytologist·Yong-Duo Sun, Svetlana Y Folimonova
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May 2, 2018·Nature Communications·Kelley ClarkWenbo Ma
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Sep 30, 2018·Planta·Poonam Kanwar, Gopaljee Jha
Nov 26, 2019·Molecular Plant-microbe Interactions : MPMI·April M MacIntyreCaitilyn Allen
Apr 27, 2021·Frontiers in Plant Science·Claudius MarondedzeChris Gehring

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Methods Mentioned

BETA
environmental stress
PCR
reverse transcription-PCR
phosphotransferase
transgenic

Software Mentioned

Adobe Photoshop
Photoshop

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