The dual role of phytoene synthase genes in carotenogenesis in carrot roots and leaves

Molecular Breeding : New Strategies in Plant Improvement
Hui WangZhen-Guo Ma

Abstract

Carrot (Daucus carota L.) is an important food crop and is useful for studying carotenogenesis due to the quantity and diversity of carotenoids in its roots. Phytoene synthase catalyzes the first committed step in the carotenoid biosynthesis pathway, and its overexpression is the main driving force in the orange phenotype. At present, we lack fundamental knowledge of the role of these genes and their effects on carotenoid accumulation in leaves. In the present study, three backcross inbred lines (BC2S4) with different colored roots derived from a cross between the orange inbred line (Af) and related wild species were used to investigate the role of the duplicated DcPSY genes in root carotenogenesis. Promoter analysis showed that DcPSY genes have diverged substantially in their regulatory sequences after gene duplication. Expression levels of DcPSY1 and DcPSY2 were generally positively correlated with carotenoid content during root development. In mature leaves, total carotenoid content was higher than that in the roots, DcPSY1 expression increased extremely higher than DcPSY2 expression compared with roots, and DcPSY1 was more sensitive than DcPSY2 during leaf de-etiolation under sunlight. These results suggest that DcPSY1 seem...Continue Reading

References

Mar 1, 1993·Plant Physiology·K Yamaguchi-Shinozaki, K Shinozaki
Dec 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·K K NiyogiA R Grossman
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·C K ShewmakerD Y Ke
Apr 20, 2001·Current Opinion in Plant Biology·J Hirschberg
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Paul D FraserPeter M Bramley
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 5, 2002·Nature Reviews. Genetics·Victoria E Prince, F Bryan Pickett
Jul 13, 2004·Plant Physiology·Cynthia E GallagherEleanore T Wurtzel
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll
Mar 14, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A V Rao, L G Rao
Aug 12, 2008·Nature·Mikihisa UmeharaShinjiro Yamaguchi
Aug 30, 2008·Journal of Experimental Botany·Maneesha AluruSteve Rodermel
Sep 2, 2008·Journal of Experimental Botany·Jérémy ClotaultEmmanuel Geoffriau
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Changfu ZhuTeresa Capell
Jun 23, 2009·Trends in Plant Science·Elizabeth A DunChristine A Beveridge
Jul 15, 2009·The Plant Journal : for Cell and Molecular Biology·Antía Rodríguez-VillalónManuel Rodríguez-Concepción
Jul 31, 2009·Plant Physiology·Alice HaywardOttoline Leyser
Aug 7, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Brian J JustPhilipp W Simon
Mar 23, 2010·Trends in Plant Science·Christopher I Cazzonelli, Barry J Pogson
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gabriela Toledo-OrtizManuel Rodríguez-Concepción
Oct 6, 2010·Plant Physiology·Cathie MartinFred Rook
Mar 13, 2012·Molecular Breeding : New Strategies in Plant Improvement·Antoine L F GadyChristian W B Bachem
May 15, 2012·The Arabidopsis Book·M Águila Ruiz-Sola, Manuel Rodríguez-Concepción
Apr 19, 2013·American Journal of Botany·Massimo IorizzoPhilipp W Simon

❮ Previous
Next ❯

Citations

Mar 29, 2020·Journal of Experimental Botany·So-Jeong JangByoung-Cheorl Kang
Jun 4, 2018·Journal of Experimental Botany·Kevin SimpsonClaudia Stange
Mar 20, 2019·Frontiers in Plant Science·Oussama AhrazemLourdes Gómez-Gómez
Aug 14, 2019·Scientific Reports·Andrew J PollackJonathan R Pollack
Jul 3, 2021·International Journal of Molecular Sciences·Tomasz OleszkiewiczRafal Baranski
Jul 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kevin M CoePhilipp Simon
Jan 12, 2016·Journal of Agricultural and Food Chemistry·Florent PerrinSéverine Gagne

❮ Previous
Next ❯

Datasets Mentioned

BETA
DQ192186
DQ192187
AB005238

Methods Mentioned

BETA
salt stress
PCR
transgenic

Software Mentioned

[UNK]
MEGA
PlantCare
NormFinder
DNAman
SAS
Primer
ClustalW
Excel
Primer Premier

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.