The Dutch Brucella abortus monitoring programme for cattle: the impact of false-positive serological reactions and comparison of serological tests

The Veterinary Quarterly
A EmmerzaalF van Zijderveld

Abstract

The Dutch national Brucella abortus eradication programme for cattle started in 1959. Sporadic cases occurred yearly until 1995; the last infected herd was culled in 1996. In August 1999 the Netherlands was declared officially free of bovine brucellosis by the European Union. Before 1999, the programme to monitor the official Brucella-free status of bovine herds was primarily based on periodical testing of dairy herds with the milk ring test (MRT) and serological testing of all animals older than 1 year of age from non-dairy herds, using the micro-agglutination test (MAT) as screening test. In addition, serum samples of cattle that aborted were tested with the MAT. The high number of false positive reactions in both tests and the serum agglutination test (SAT) and complement fixation test (CFT) used for confirmation seemed to result in unnecessary blockade of herds, subsequent testing and slaughter of animals. For this reason, a validation study was performed in which three indirect enzyme-linked immunosorbent assays (ELISAs), the CFT and the SAT were compared using a panel of sera from brucellosis-free cattle, sera from experimentally infected cattle, and sera from cattle experimentally infected with bacteria which are known t...Continue Reading

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Citations

Dec 28, 2011·Tropical Animal Health and Production·Amel Abdalla, Mohamed E Hamid
Aug 19, 2010·Croatian Medical Journal·Jacques GodfroidClaude Saegerman
May 1, 2007·Research in Veterinary Science·J McGivenI Mawhinney
Dec 26, 2012·Pathogens and Global Health·Lisa D SpragueHeinrich Neubauer
Feb 10, 2006·BMC Bioinformatics·Kim W CarterLyle J Palmer
Apr 25, 2019·Tropical Medicine and Infectious Disease·Johanna F LindahlManish Kakkar
Jun 24, 2017·PLoS Neglected Tropical Diseases·Jiin FelgnerD Huw Davies

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