PMID: 11607708Sep 17, 1996Paper

The dwarf-1 (dt) Mutant of Zea mays blocks three steps in the gibberellin-biosynthetic pathway

Proceedings of the National Academy of Sciences of the United States of America
C R SprayJ Macmillan

Abstract

In plants, gibberellin (GA)-responding mutants have been used as tools to identify the genes that control specific steps in the GA-biosynthetic pathway. They have also been used to determine which native GAs are active per se, i.e., further metabolism is not necessary for bioactivity. We present metabolic evidence that the D1 gene of maize (Zea mays L.) controls the three biosynthetic steps: GA20 to GA1, Ga20 to GA5, and GA5 to GA3. We also present evidence that three gibberellins, GA1, GA5, and GA3, have per se activity in stimulating shoot elongation in maize. The metabolic evidence comes from the injection of [17-13C,3H]GA20 and [17-13C,3H]GA5 into seedlings of d1 and controls (normal and d5), followed by isolation and identification of the 13C-labeled metabolites by full-scan GC-MS and Kovats retention index. For the controls, GA20 was metabolized to GA1,GA3, and GA5; GA5 was metabolized to GA3. For the d1 mutant, GA20 was not metabolized to GA1, GA3, or to GA5, and GA5 was not metabolized to GA3. The bioassay evidence is based on dosage response curves using d1 seedlings for assay. GA1, GA3, and GA5 had similar bioactivities, and they were 10-times more active than GA20.

References

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Citations

Sep 15, 2005·Planta·Nigel E J ApplefordPeter Hedden
Nov 12, 2013·Plant Molecular Biology·Hongkun LvGuoying Wang
Dec 17, 2002·Phytochemistry·Tania S StokesDavid E Hanke
Dec 12, 2002·Phytochemistry·Rod W KingBruce Twitchin
Dec 20, 2000·Trends in Plant Science·P Hedden, A L Phillips
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·U HoeckerD R McCarty
Sep 2, 2005·The Journal of Heredity·D L AugerJ A Birchler
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Peter Hedden, Yuji Kamiya
Jan 5, 2008·Annual Review of Plant Biology·Shinjiro Yamaguchi
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H ItohM Kobayashi
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·D N MartinP Hedden
Jun 4, 2015·Plant Signaling & Behavior·Yi Chen, Bao-Cai Tan
Sep 4, 2007·The Plant Journal : for Cell and Molecular Biology·Bjorn KloostermanChristian W B Bachem
Oct 3, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Adriana Garay-ArroyoElena R Alvarez-Buylla
Apr 27, 2012·The Biochemical Journal·Peter Hedden, Stephen G Thomas
Apr 1, 2014·Current Opinion in Plant Biology·Jisen ZhangRay Ming
Oct 30, 2016·Frontiers in Plant Science·Ashley R Smith, Dazhong Zhao
May 28, 2008·Cytogenetic and Genome Research·M JamilenaS Manzano
Oct 2, 2012·The Plant Journal : for Cell and Molecular Biology·Feng TengZuxin Zhang
Jun 13, 2020·Frontiers in Plant Science·Ziming MaWenzhu Jiang
Oct 17, 2018·International Journal of Molecular Sciences·Chenggang XiangChanglin Wang
Jan 8, 2020·International Journal of Molecular Sciences·Haileslassie GebremeskelWenge Liu
Aug 14, 1998·Plant Physiology·R J CowlingN P Harberd

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