The dynamic adaptation of primary human endothelial cells to simulated microgravity

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Alessandra CazzanigaJeanette A M Maier

Abstract

Culture of human endothelial cells for 10 d in real microgravity onboard the International Space Station modulated more than 1000 genes, some of which are involved in stress response. On Earth, 24 h after exposure to simulated microgravity, endothelial cells up-regulate heat shock protein (HSP) 70. To capture a broad view of endothelial stress response to gravitational unloading, we cultured primary human endothelial cells for 4 and 10 d in the rotating wall vessel, a U.S. National Aeronautics and Space Administration-developed surrogate system for benchtop microgravity research on Earth. We highlight the crucial role of the early increase of HSP70 because its silencing markedly impairs cell survival. Once HSP70 up-regulation fades away after 4 d of simulated microgravity, a complex and articulated increase of various stress proteins (sirtuin 2, paraoxonase 2, superoxide dismutase 2, p21, HSP27, and phosphorylated HSP27, all endowed with cytoprotective properties) occurs and counterbalances the up-regulation of the pro-oxidant thioredoxin interacting protein (TXNIP). Interestingly, TXNIP was the most overexpressed transcript in endothelial cells after spaceflight. We conclude that HSP70 up-regulation sustains the initial adapti...Continue Reading

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Citations

May 6, 2019·Antioxidants·Débora LevySérgio Paulo Bydlowski
Dec 19, 2019·Pharmaceutical Research·Lucie A Low, Marc A Giulianotti
Jul 28, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dominique Thuringer, Carmen Garrido
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Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura LocatelliJeanette A M Maier
Dec 2, 2020·International Journal of Molecular Sciences·Alessandra CazzanigaJeanette A Maier
Dec 16, 2020·International Journal of Molecular Sciences·Binod PrasadMarcus Krüger
Jul 6, 2021·Frontiers in Bioengineering and Biotechnology·Stefan RiwaldtDaniela Grimm
Sep 28, 2021·Frontiers in Cell and Developmental Biology·Laura Locatelli, Jeanette A M Maier
Oct 30, 2021·Cellular and Molecular Life Sciences : CMLS·Francesca CialdaiMatteo Lulli
Jan 18, 2022·Expert Review of Proteomics·Herbert SchulzDaniela Grimm

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