The dynamic changes of T-bet(+)/GATA-3(+) and RORgammat(+)/FOXP3(+) cells in recipient spleens and grafts after rat orthotopic liver transplantation

Transplant Immunology
Min XuJia Fan

Abstract

Rejection of transplanted liver occurs when the host generates alloantigen-reactive T cells, and CD4(+) T-cell subsets, including Th1, Th2, T17 and iTreg, could be involved in changing the dynamics of graft rejection onset. In the current immunosuppressive strategies, rejection is treated as an undifferentiated process that is uniform, which results in the failure of tolerance induction. Here, we established a rejection model to observe the reciprocal interaction of CD4(+) T-cell subsets in the complex networks of the immune system. Orthotopic liver transplantation (OLT) from male inbred Lewis rats (n=15) to male inbred Brown Norway (BN) rats was performed by Kamada's two-cuff technique without reconstruction of the hepatic artery. OLT from BN to BN rats (n=5) was performed as a control. Recipients were sacrificed on postoperative days 3, 5, 7, 9, 11, 13 and 15. Recipient spleens and grafts were harvested, fixed in 10% neutral formalin, and embedded in paraffin. Meanwhile, hematoxylin and eosin and immunohistochemical staining was done, acute rejection was graded by the Banff scheme, and the number of T-bet(+), GATA-3(+), RORgammat(+) and FOXP3(+) cells in the spleen and grafts were counted. In recipient spleens, the T-bet(+) a...Continue Reading

References

Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Oct 1, 1993·The Journal of Pathology·G CattorettiE Feudale
Jun 1, 1996·The Journal of Experimental Medicine·F FossiezS Lebecque
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·A BoehlerS Keshavjee
Mar 29, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J HjelmesaethP Fauchald
Mar 22, 2002·Nature Reviews. Immunology·T E Starzl, R M Zinkernagel
Jul 13, 2002·The Journal of Pathology·Che-Chuan LoongChing-Yuang Lin
Jan 8, 2003·The Journal of Experimental Medicine·Kevin J MaloyFiona Powrie
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Nov 17, 2004·The Journal of Clinical Investigation·Patrick T WalshLaurence A Turka
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yisong Y Wan, Richard A Flavell
Jun 20, 2006·Immunity·Casey T WeaverKenneth M Murphy
Jun 27, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Barbara S HöroldtJames M Neuberger
Oct 25, 2006·Xenotransplantation·Ming-Lee LinAndrew M Lew
Dec 13, 2006·European Journal of Immunology·Jun WangRené E M Toes
Dec 23, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vinodh PillaiNitin J Karandikar
Nov 13, 2007·International Immunopharmacology·Heather KopfXin Chen

❮ Previous
Next ❯

Citations

Nov 1, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Min XuWilliam C Chapman
Sep 22, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R TaubertE Jaeckel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Autoimmune Hepatitis

Autoimmune hepatitis formerly called lupoid hepatitis, is a chronic, autoimmune disease of the liver that occurs when the body's immune system attacks liver cells causing the liver to be inflamed. Discover the latest research on autoimmune hepatitis here.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Annette ErhardtGisa Tiegs
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Barbara S HöroldtJames M Neuberger
Human Molecular Genetics
Adam G West, Peter Fraser
Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Yong ChenJ Gong
© 2022 Meta ULC. All rights reserved