Abstract
Rejection of transplanted liver occurs when the host generates alloantigen-reactive T cells, and CD4(+) T-cell subsets, including Th1, Th2, T17 and iTreg, could be involved in changing the dynamics of graft rejection onset. In the current immunosuppressive strategies, rejection is treated as an undifferentiated process that is uniform, which results in the failure of tolerance induction. Here, we established a rejection model to observe the reciprocal interaction of CD4(+) T-cell subsets in the complex networks of the immune system. Orthotopic liver transplantation (OLT) from male inbred Lewis rats (n=15) to male inbred Brown Norway (BN) rats was performed by Kamada's two-cuff technique without reconstruction of the hepatic artery. OLT from BN to BN rats (n=5) was performed as a control. Recipients were sacrificed on postoperative days 3, 5, 7, 9, 11, 13 and 15. Recipient spleens and grafts were harvested, fixed in 10% neutral formalin, and embedded in paraffin. Meanwhile, hematoxylin and eosin and immunohistochemical staining was done, acute rejection was graded by the Banff scheme, and the number of T-bet(+), GATA-3(+), RORgammat(+) and FOXP3(+) cells in the spleen and grafts were counted. In recipient spleens, the T-bet(+) a...Continue Reading
References
Oct 1, 1991·The Journal of Experimental Medicine·R de Waal MalefytJ E de Vries
Jun 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiK L Kalra
Oct 1, 1993·The Journal of Pathology·G CattorettiE Feudale
Jan 27, 1995·Transplantation·N L ThaiR J Duquesnoy
May 30, 1996·Nature·C P LarsenT C Pearson
Jun 1, 1996·The Journal of Experimental Medicine·F FossiezS Lebecque
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases
May 16, 1997·Cell·W Zheng, R A Flavell
Jul 22, 1998·The Journal of Experimental Medicine·A M Thornton, E M Shevach
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·A BoehlerS Keshavjee
Jun 9, 1999·Transplantation·G H RingF G Lakkis
Apr 13, 2000·Cell·S J SzaboL H Glimcher
Mar 29, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·J HjelmesaethP Fauchald
Jun 8, 2001·The Journal of Experimental Medicine·H JonuleitA H Enk
Jun 8, 2001·The Journal of Experimental Medicine·D DieckmannG Schuler
Jun 9, 2001·Science·A C MullenS L Reiner
Jul 27, 2001·Transplantation·C WangG A Bishop
Aug 22, 2001·The Journal of Experimental Medicine·P YeJ K Kolls
Jan 12, 2002·Science·Susanne J SzaboLaurie H Glimcher
Mar 22, 2002·Nature Reviews. Immunology·T E Starzl, R M Zinkernagel
Jul 13, 2002·The Journal of Pathology·Che-Chuan LoongChing-Yuang Lin
Jan 8, 2003·The Journal of Experimental Medicine·Kevin J MaloyFiona Powrie
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 5, 2003·European Journal of Immunology·Kajsa WingElisabeth Suri-Payer
Nov 17, 2004·The Journal of Clinical Investigation·Patrick T WalshLaurence A Turka
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yisong Y Wan, Richard A Flavell
Jun 20, 2006·Immunity·Casey T WeaverKenneth M Murphy
Jun 27, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Barbara S HöroldtJames M Neuberger
Sep 23, 2006·Cell·Ivaylo I IvanovDan R Littman
Oct 25, 2006·Xenotransplantation·Ming-Lee LinAndrew M Lew
Dec 13, 2006·European Journal of Immunology·Jun WangRené E M Toes
Dec 23, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Vinodh PillaiNitin J Karandikar
Aug 10, 2007·Allergy·C MalmhällJ Lötvall
Nov 13, 2007·International Immunopharmacology·Heather KopfXin Chen
Citations
Nov 1, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Min XuWilliam C Chapman
Aug 11, 2011·PloS One·Fay Lin KhongDarren J Kelly
Mar 8, 2013·Transplantation·Jie HuJian Zhou
Aug 23, 2016·Transplantation·Stanley C JordanAshley Vo
Sep 22, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R TaubertE Jaeckel
Jan 1, 2021·Frontiers in Immunology·Fengyi XiaoSun-Wei Guo