The dynamic relationships between the three events that release individual Na⁺ ions from the Na⁺/K⁺-ATPase.

Nature Communications
D C GadsbyM Holmgren

Abstract

Na(+)/K(+) pumps move net charge through the cell membrane by mediating unequal exchange of intracellular Na(+) and extracellular K(+). Most charge moves during transitions that release Na(+) to the cell exterior. When pumps are constrained to bind and release only Na(+), a membrane voltage-step redistributes pumps among conformations with zero, one, two or three bound Na(+), thereby transiently generating current. By applying rapid voltage steps to squid giant axons, we previously identified three components in such transient currents, with distinct relaxation speeds: fast (which nearly parallels the voltage-jump time course), medium speed (τ(m)=0.2-0.5 ms) and slow (τ(s)=1-10 ms). Here we show that these three components are tightly correlated, both in their magnitudes and in the time courses of their changes. The correlations reveal the dynamics of the conformational rearrangements that release three Na(+) to the exterior (or sequester them into their binding sites) one at a time, in an obligatorily sequential manner.

References

Aug 9, 1979·Nature·L A Beaugé, I M Glynn
Apr 1, 1991·The Journal of Membrane Biology·W StürmerP Läuger
Jan 1, 1990·Annual Review of Biochemistry·I M Glynn, S J Karlish
Oct 16, 1986·Nature·M Nakao, D C Gadsby
Apr 1, 1987·The Journal of General Physiology·J R StimersR E Taylor
Aug 1, 1994·The Journal of General Physiology·S HeyseW Stürmer
Jan 1, 1993·The Journal of General Physiology·R F Rakowski
Aug 10, 2004·Biophysical Journal·R Daniel Peluffo
Dec 14, 2007·Nature·Claus OlesenPoul Nissen
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chikashi ToyoshimaHaruo Ogawa
Jan 1, 1967·Annual Review of Biochemistry·R W Albers
Jul 1, 1969·The Journal of General Physiology·R L PostA K Sen

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Citations

Apr 2, 2014·The Journal of General Physiology·Donald W Hilgemann
Apr 2, 2014·The Journal of General Physiology·Natascia Vedovato, David C Gadsby
Oct 18, 2015·The FEBS Journal·Michael Voldsgaard ClausenHanne Poulsen
Nov 2, 2014·The Journal of Physiology·Alexandre LewalleNicolas P Smith
Oct 22, 2015·Biophysical Journal·Ronald J Clarke
Jan 11, 2014·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Fernando A F BrazSérgio V A Campos
Jul 16, 2013·Biochimica Et Biophysica Acta·Ronald J ClarkeHans-Jürgen Apell
Sep 6, 2012·Biophysical Journal·Alvaro GarciaRonald J Clarke
Jul 28, 2016·Journal of the American Chemical Society·Takashi Sumikama, Shigetoshi Oiki
Oct 22, 2016·Journal of Theoretical Biology·A G Dimitrov
Jan 14, 2017·Scientific Reports·Minwoo HanHimanshu Khandelia
Jan 25, 2017·Biochimica Et Biophysica Acta. Biomembranes·Alvaro GarciaRonald J Clarke
May 17, 2018·The Journal of Biological Chemistry·Olga N RaguimovaSeth L Robia
Feb 21, 2019·Advanced Materials·Xiaoxu ZhaoKian Ping Loh
Jul 25, 2015·Nature Communications·Juan P CastilloMiguel Holmgren
Jul 18, 2019·The Journal of General Physiology·Olaf S AndersenRobert M Shapley
Jun 27, 2018·The Journal of General Physiology·Francisco Bezanilla
Aug 26, 2018·Scientific Reports·Vikas DubeyHimanshu Khandelia
Aug 25, 2015·Neuropathology and Applied Neurobiology·Mona SadeghianKenneth J Smith
Nov 25, 2020·The Journal of General Physiology·Dylan J MeyerPablo Artigas
Dec 13, 2018·Journal of Molecular Biology·Huan RuiBenoît Roux
Apr 4, 2021·International Journal of Molecular Sciences·Anna ZimnaKatarzyna Maria Marzec
Apr 4, 2015·Biochemistry·Robert V Grădinaru, Hans-Jürgen Apell
Sep 23, 2021·Faraday Discussions·Khondker R HossainRonald J Clarke

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