The dynamic steroid landscape of equine pregnancy mapped by mass spectrometry

Reproduction : the Official Journal of the Society for the Study of Fertility
Erin L LegackiAlan J Conley

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) allowed comprehensive analysis of various steroids detectable in plasma throughout equine gestation. Mares (n=9) were bled serially until they foaled. Certain steroids dominated the profile at different stages of gestation, clearly defining key physiological and developmental transitions. The period (weeks 6-20) coincident with equine chorionic gonadotropic (eCG) stimulation of primary corpora lutea and subsequent formation of secondary luteal structures was defined by increased progesterone, 17OH-progesterone and androstenedione, all Δ4 steroids. The 5α-reduced metabolite of progesterone, dihydroprogesterone (DHP) paralleled progesterone secretion at less than half the concentration until week 12 of gestation when progesterone began to decline but DHP concentrations continued to increase. DHP exceeded progesterone concentrations by week 16, clearly defining the luteo-placental shift in pregnane synthesis from primarily ovarian to primarily placental. The period corresponding to the growth of fetal gonads was defined by increasing dehydroepiandrosterone and pregnenolone (Δ5 steroids) concentrations from week 14, peaking at week 34 and declining to term. Metabolites of DH...Continue Reading

References

Jan 1, 1975·Journal of Steroid Biochemistry·C R ParkerV B Mahesh
Mar 1, 1976·Journal of Reproduction and Fertility·J I Raeside
Nov 15, 1976·FEBS Letters·B K ParkP D Dean
Feb 1, 1975·Journal of Animal Science·D W HoltanV L Estergreen
Feb 1, 1975·Journal of Animal Science·E L Squires, O J Ginther
Sep 1, 1990·Journal of Reproduction and Fertility·P F DaelsG H Stabenfeldt
Jul 1, 1967·The Journal of Endocrinology·E MacArthurV J O'Donnell
Oct 1, 1973·Journal of Animal Science·T M NettV Liné Estergreen
Feb 1, 1974·Journal of Animal Science·E L SquiresO J Ginther
Apr 1, 1969·The Journal of Endocrinology·W R Allen
May 1, 1980·Biology of Reproduction·J A Wesson, O J Ginther
Jan 1, 1994·Experimental and Clinical Endocrinology·E Möstl
Sep 1, 1995·Equine Veterinary Journal·J I Raeside
Oct 22, 1998·Biology of Reproduction·P F DaelsH O Mohammed
Dec 1, 1959·The Journal of Endocrinology·R V SHORT
Nov 29, 2005·Neuroscience·D EserR Rupprecht
Apr 15, 1996·Theriogenology·P F DaelsH O Mohammed
Jun 23, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Martin HillLuboslav Stárka
Aug 21, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benjamin C Moeller, Scott D Stanley
Sep 10, 2013·Progress in Neurobiology·Paula J BruntonJonathan J Hirst
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth L ScholtzAlan J Conley

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Citations

Jul 29, 2016·The Journal of Endocrinology·C J CorbinA J Conley
Apr 19, 2018·Biology of Reproduction·Lawrence P ReynoldsAlan J Conley
Oct 27, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Erin L LegackiAlan J Conley
Sep 8, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Erin L LegackiAlan J Conley
Jan 18, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Michelle A A WynnMats Troedsson
Feb 15, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Alan J ConleyLawrence P Reynolds
Jun 2, 2018·The Journal of Endocrinology·A J ConleyS D Stanley
Aug 29, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Erin L LegackiA J Conley
Feb 24, 2020·Theriogenology·Igor F CanissoFabio S Lima
Nov 17, 2020·Frontiers in Veterinary Science·Gianfranco GabaiLieta Marinelli
Apr 7, 2021·The Veterinary Clinics of North America. Equine Practice·Morgane RoblesPascale Chavatte-Palmer

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