The dynamic transmission of positional information in stau- mutants during Drosophila embryogenesis.

ELife
Zhe YangFeng Liu

Abstract

It has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, the mechanism that underlies this control is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With improved control of measurement errors, we show that the xHb of stau- mutants reproducibly moves posteriorly by 10% of the embryo length (EL) to the wild type (WT) position in the nuclear cycle (nc) 14, and that its variability over short time windows is comparable to that of the WT. Moreover, for stau- mutants, the upstream Bicoid (Bcd) gradients show equivalent relative intensity noise to that of the WT in nc12-nc14, and the downstream Even-skipped (Eve) and cephalic furrow (CF) show the same positional errors as these factors in WT. Our results indicate that threshold-dependent activation and self-organized filtering are not mutually exclusive and could both be implemented in early Drosophila embryogenesis.

References

Jul 12, 1991·Cell·D St JohnstonC Nüsslein-Volhard
Oct 1, 1969·Journal of Theoretical Biology·L Wolpert
Feb 15, 2002·Nature·Bahram HouchmandzadehStanislas Leibler
Jul 16, 2004·Nature·Johannes JaegerJohn Reinitz
Nov 8, 2005·Current Biology : CB·Olivier Crauk, Nathalie Dostatni
Oct 17, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Johannes Jaeger, John Reinitz
Feb 15, 2007·PLoS Biology·Sven BergmannNaama Barkai
Jul 17, 2007·Cell·Thomas GregorDavid W Tank
Jul 17, 2007·Cell·Thomas GregorWilliam Bialek
Apr 3, 2008·FEBS Letters·Andrew B Goryachev, Alexandra V Pokhilko
Sep 27, 2008·PLoS Computational Biology·Francisco J P LopesAlexander V Spirov
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jitendra S KanodiaStanislav Y Shvartsman
Mar 12, 2010·Current Biology : CB·Aude Porcher, Nathalie Dostatni
Jun 24, 2010·Development·Oliver GrimmEric Wieschaus
Aug 5, 2010·Biophysical Journal·Feng HeJun Ma
Oct 12, 2010·Cellular and Molecular Life Sciences : CMLS·Johannes Jaeger
Nov 4, 2010·Journal of Theoretical Biology·Lewis Wolpert
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Katherine W Rogers, Alexander F Schier
Mar 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Francis Corson, Eric Dean Siggia
Jun 5, 2012·Nature Methods·Uros KrzicLars Hufnagel
Jan 24, 2013·Molecular Systems Biology·Julien O DubuisThomas Gregor
Apr 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Feng LiuThomas Gregor
Oct 22, 2013·Current Biology : CB·Tanguy LucasNathalie Dostatni
Feb 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Andrew R Bassett, Ji-Long Liu
May 27, 2014·Current Biology : CB·Mariela D PetkovaThomas Gregor
Jan 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Max V StallerAngela H DePace
Nov 3, 2015·Nature Methods·Idse Heemskerk, Sebastian J Streichan
Mar 13, 2016·Current Topics in Developmental Biology·Lewis Wolpert
Feb 6, 2017·PLoS Computational Biology·Berta VerdJohannes Jaeger

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging technique

Software Mentioned

ImSAnE ( Image Surface Analysis Environment )
MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.