The Dynamics of Transcript Abundance during Cellularization of Developing Barley Endosperm

Plant Physiology
Runxuan ZhangGeoffrey B Fincher

Abstract

Within the cereal grain, the endosperm and its nutrient reserves are critical for successful germination and in the context of grain utilization. The identification of molecular determinants of early endosperm development, particularly regulators of cell division and cell wall deposition, would help predict end-use properties such as yield, quality, and nutritional value. Custom microarray data have been generated using RNA isolated from developing barley grain endosperm 3 d to 8 d after pollination (DAP). Comparisons of transcript abundance over time revealed 47 gene expression modules that can be clustered into 10 broad groups. Superimposing these modules upon cytological data allowed patterns of transcript abundance to be linked with key stages of early grain development. Here, attention was focused on how the datasets could be mined to explore and define the processes of cell wall biosynthesis, remodeling, and degradation. Using a combination of spatial molecular network and gene ontology enrichment analyses, it is shown that genes involved in cell wall metabolism are found in multiple modules, but cluster into two main groups that exhibit peak expression at 3 DAP to 4 DAP and 5 DAP to 8 DAP. The presence of transcription f...Continue Reading

Citations

May 17, 2017·The Plant Journal : for Cell and Molecular Biology·Natalie S BettsGeoffrey B Fincher
Oct 20, 2019·The Plant Journal : for Cell and Molecular Biology·Lim Chee LiewMathew G Lewsey
Jun 13, 2020·The Plant Journal : for Cell and Molecular Biology·Christian HertigJohannes Thiel
Nov 30, 2019·Plant Biotechnology Journal·Lei ChenPaula E Jameson
Aug 27, 2019·Frontiers in Plant Science·Vincent BuloneGeoffrey B Fincher
Nov 5, 2019·Frontiers in Plant Science·Bryan W PenningNicholas C Carpita
Mar 18, 2021·Frontiers in Plant Science·Helen M CollinsGeoffrey B Fincher

Related Concepts

Hordeum vulgare
Cell Wall
Triticale
Plant Proteins
Disease Clustering
Gene Expression Regulation, Plant
Gene Expression Regulation, Developmental
Cdna Microarrays
MRNA Differential Display
Gene Modules