The Dysregulated Expression of KCNQ1OT1 and Its Interaction with Downstream Factors miR-145/CCNE2 in Breast Cancer Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Weiliang FengMing Sun

Abstract

Next-generation sequencing (NGS) has revealed abundant long noncoding RNAs (lncRNAs) that have been characterized as critical components of cancer biology in humans. The present study aims to investigate the role of the lncRNA KCNQ1OT1 in breast cancer (BRCA) as well as the underlying molecular mechanisms and functions of KCNQ1OT1 involved in the progression of BRCA. The Cancer Genome Atlas (TCGA) and StarBase v2.0 were used to obtain the required gene data. Dual luciferase reporter gene assays were conducted to verify the relevant intermolecular target relationships. QRT-PCR and Western blot were performed to measure the expression levels of different molecules. Cell proliferation was detected by using the MTT and colony formation assays, while cell migration and invasion were examined by transwell assay. Variations in cell apoptosis and cell cycle were determined through flow cytometry. A tumor xenograft model was applied to assess tumor growth in vivo. KCNQ1OT1 was found to be remarkably highly expressed in BRCA tissues and cells. KCNQ1OT1 modulated CCNE2 through sponging miR-145 in BRCA. KCNQ1OT1 promoted tumor growth in vivo by regulating miR-145/CCNE2. The KCNQ1OT1/miR-145/CCNE2 axis plays a critical regulatory role in BR...Continue Reading

Citations

Apr 16, 2019·Technology in Cancer Research & Treatment·Tianzhu ZhangZhongxin Lu
Jun 8, 2019·Molecular Medicine Reports·Zhen DengJianming Tan
Aug 9, 2019·In Vitro Cellular & Developmental Biology. Animal·Meng WangZhibo Liu
Nov 24, 2020·Future Virology·Rafeed Rahman TurjyaAbul Bashar Mir Md Khademul Islam
Jul 13, 2020·Current Cancer Drug Targets·Ravishkumar L AkshayaNagarajan Selvamurugan
Sep 10, 2021·Frontiers in Cell and Developmental Biology·Weipeng ZhaoZhongsheng Tong
Oct 12, 2021·Frontiers in Genetics·Junjie LiuJingwen Deng

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