Molecular self-organziation, also regarded as pattern formation, is crucial for the correct distribution of cellular content. The processes leading to spatiotemporal patterns often involve a multitude of molecules interacting in complex networks, so that only very few cellular pattern-forming systems can be regarded as well understood. Due to its compositional simplicity, the Escherichia coli MinCDE system has, thus, become a paradigm for protein pattern formation. This biological reaction diffusion system spatiotemporally positions the division machinery in E. coli and is closely related to ParA-type ATPases involved in most aspects of spatiotemporal organization in bacteria. The ATPase MinD and the ATPase-activating protein MinE self-organize on the membrane as a reaction matrix. In vivo, these two proteins typically oscillate from pole-to-pole, while in vitro they can form a variety of distinct patterns. MinC is a passenger protein supposedly operating as a downstream cue of the system, coupling it to the division machinery. The MinCDE system has helped to extract not only the principles underlying intracellular patterns, but also how they are shaped by cellular boundaries. Moreover, it serves as a model to investigate how p...Continue Reading
Roles of MinC and MinD in the site-specific septation block mediated by the MinCDE system of Escherichia coli
The Escherichia coli minB mutation resembles gyrB in defective nucleoid segregation and decreased negative supercoiling of plasmids
A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli
Proper placement of the Escherichia coli division site requires two functions that are associated with different domains of the MinE protein
Isolation of an ftsZ homolog from the archaebacterium Halobacterium salinarium: implications for the evolution of FtsZ and tubulin
Deletion analysis of gene minE which encodes the topological specificity factor of cell division in Escherichia coli
Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli
Structure of ADP x AIF4(-)-stabilized nitrogenase complex and its implications for signal transduction
The Escherichia coli histone-like protein HU affects DNA initiation, chromosome partitioning via MukB, and cell division via MinCDE
The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli
Dominant C-terminal deletions of FtsZ that affect its ability to localize in Caulobacter and its interaction with FtsA
FtsZ from Escherichia coli, Azotobacter vinelandii, and Thermotoga maritima--quantitation, GTP hydrolysis, and assembly
The relationship between hetero-oligomer formation and function of the topological specificity domain of the Escherichia coli MinE protein
Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site
Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli
Selection of the midcell division site in Bacillus subtilis through MinD-dependent polar localization and activation of MinC
Topological regulation of cell division in Escherichia coli involves rapid pole to pole oscillation of the division inhibitor MinC under the control of MinD and MinE
The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
Dynamic movement of the ParA-like Soj protein of B. subtilis and its dual role in nucleoid organization and developmental regulation
Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast
The bacterial cell-division protein ZipA and its interaction with an FtsZ fragment revealed by X-ray crystallography
The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle
Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus
Topological regulation of cell division in E. coli. spatiotemporal oscillation of MinD requires stimulation of its ATPase by MinE and phospholipid
Gonococcal MinD affects cell division in Neisseria gonorrhoeae and Escherichia coli and exhibits a novel self-interaction
Pattern formation in Escherichia coli: a model for the pole-to-pole oscillations of Min proteins and the localization of the division site
Targeting of (D)MinC/MinD and (D)MinC/DicB complexes to septal rings in Escherichia coli suggests a multistep mechanism for MinC-mediated destruction of nascent FtsZ rings
Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains
The role of co-transcriptional translation and protein translocation (transertion) in bacterial chromosome segregation
Conservation of dynamic localization among MinD and MinE orthologues: oscillation of Neisseria gonorrhoeae proteins in Escherichia coli
Membrane localization of MinD is mediated by a C-terminal motif that is conserved across eubacteria, archaea, and chloroplasts
Recruitment of MinC, an inhibitor of Z-ring formation, to the membrane in Escherichia coli: role of MinD and MinE
A conserved sequence at the C-terminus of MinD is required for binding to the membrane and targeting MinC to the septum
ATP-dependent interactions between Escherichia coli Min proteins and the phospholipid membrane in vitro
Concentration and assembly of the division ring proteins FtsZ, FtsA, and ZipA during the Escherichia coli cell cycle
Membrane binding by MinD involves insertion of hydrophobic residues within the C-terminal amphipathic helix into the bilayer
Mapping the MinE site involved in interaction with the MinD division site selection protein of Escherichia coli
Positioning of the MinE binding site on the MinD surface suggests a plausible mechanism for activation of the Escherichia coli MinD ATPase during division site selection
Analysis of MinD mutations reveals residues required for MinE stimulation of the MinD ATPase and residues required for MinC interaction
SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli
Entropy-driven spatial organization of highly confined polymers: lessons for the bacterial chromosome
Amphipathic helices as mediators of the membrane interaction of amphitropic proteins, and as modulators of bilayer physical properties
Bacterially derived 400 nm particles for encapsulation and cancer cell targeting of chemotherapeutics
The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP
The Min system as a general cell geometry detection mechanism: branch lengths in Y-shaped Escherichia coli cells affect Min oscillation patterns and division dynamics
Mutual effects of MinD-membrane interaction: II. Domain structure of the membrane enhances MinD binding
Mutual effects of MinD-membrane interaction: I. Changes in the membrane properties induced by MinD binding
A novel component of the division-site selection system of Bacillus subtilis and a new mode of action for the division inhibitor MinCD
Bacillus subtilis MinC destabilizes FtsZ-rings at new cell poles and contributes to the timing of cell division
The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD
A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation
Appropriation of the MinD protein-interaction motif by the dimeric interface of the bacterial cell division regulator MinE
Determination of the structure of the MinD-ATP complex reveals the orientation of MinD on the membrane and the relative location of the binding sites for MinE and MinC
The N-terminal amphipathic helix of the topological specificity factor MinE is associated with shaping membrane curvature
The Min oscillator uses MinD-dependent conformational changes in MinE to spatially regulate cytokinesis
Cellular architecture mediates DivIVA ultrastructure and regulates min activity in Bacillus subtilis
Polar flagellar biosynthesis and a regulator of flagellar number influence spatial parameters of cell division in Campylobacter jejuni
The comparison of the aging male symptoms (AMS) scale and androgen deficiency in the aging male (ADAM) questionnaire to detect androgen deficiency in middle-aged men
Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli
Highly canalized MinD transfer and MinE sequestration explain the origin of robust MinCDE-protein dynamics
MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli.
Reconstitution of pole-to-pole oscillations of min proteins in microengineered polydimethylsiloxane compartments
Engineering the type III secretion system in non-replicating bacterial minicells for antigen delivery
Differential affinities of MinD and MinE to anionic phospholipid influence Min patterning dynamics in vitro
Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinD
Quantitative Proteomics Analysis Reveals the Min System of Escherichia coli Modulates Reversible Protein Association with the Inner Membrane.
Complex polar machinery required for proper chromosome segregation in vegetative and sporulating cells of Bacillus subtilis
Chemophoresis as a driving force for intracellular organization: Theory and application to plasmid partitioning
GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis
Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence
Characterization of C-terminal structure of MinC and its implication in evolution of bacterial cell division
Dissecting the role of conformational change and membrane binding by the bacterial cell division regulator MinE in the stimulation of MinD ATPase activity
The Min-protein oscillations in Escherichia coli : an example of self-organized cellular protein waves
A cell length-dependent transition in MinD-dynamics promotes a switch in division-site placement and preservation of proliferating elongated Vibrio parahaemolyticus swarmer cells
The Min Oscillator Defines Sites of Asymmetric Cell Division in Cyanobacteria during Stress Recovery
MinC N- and C-Domain Interactions Modulate FtsZ Assembly, Division Site Selection, and MinD-Dependent Oscillation in Escherichia coli
Integrated Proteomic and Transcriptomic Analyses Reveal the Roles of Brucella Homolog of BAX Inhibitor 1 in Cell Division and Membrane Homeostasis of Brucella suis S2.
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