The E3 ubiquitin ligase activity of arabidopsis PLANT U-BOX17 and its functional tobacco homolog ACRE276 are required for cell death and defense

The Plant Cell
Chengwei YangAri Sadanandom

Abstract

Previous analysis of transcriptional changes after elicitation of Cf-9 transgenic tobacco (Nicotiana tabacum) by Avr9 peptide revealed a rapidly upregulated gene, ACRE276. We show that ACRE276 is transiently induced in wounded leaves within 15 min, but upon Avr9 elicitor treatment, this upregulation is enhanced and maintained until cell death onset in Cf-9 tobacco. ACRE276 RNA interference (RNAi) silencing in tobacco results in loss of hypersensitive response (HR) specified by Cf resistance genes. ACRE276 RNAi plants are also compromised for HR mediated by the tobacco mosaic virus defense elicitor p50. Silencing tomato (Lycopersicon esculentum) ACRE276 leads to breakdown of Cf-9-specified resistance against Cladosporium fulvum leaf mold. We confirmed that tobacco ACRE276 is an E3 ubiquitin ligase requiring an intact U-box domain. Bioinformatic analyses revealed Arabidopsis thaliana PLANT U-BOX17 (PUB17) and Brassica napus ARC1 as the closest homologs of tobacco ACRE276. Transiently expressing PUB17 in Cf-9 tobacco silenced for ACRE276 restores HR, while mutant PUB17 lacking E3 ligase activity fails to do so, demonstrating that PUB17 ligase activity is crucial for defense signaling. Arabidopsis PUB17 knockout plants are compromi...Continue Reading

References

Jun 1, 1992·The Plant Cell·S UknesJ Ryals
Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·R B HorschR T Fraley
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·D C SchererD W Ballard
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·T GuD R Goring
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Dec 19, 1998·The EMBO Journal·A Ciechanover
May 26, 1999·The Plant Journal : for Cell and Molecular Biology·F L EricksonB Baker
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·K L LorickA M Weissman
Mar 8, 2000·Current Biology : CB·L Aravind, E V Koonin
Oct 6, 2000·Current Opinion in Plant Biology·J Callis, R D Vierstra
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·C KirschK Hahlbrock
Jul 4, 2001·The Journal of Biological Chemistry·S HatakeyamaK I Nakayama
Jul 19, 2001·Nature·J L Dangl, J D Jones
Aug 10, 2001·Trends in Plant Science·C AzevedoK Shirasu
Sep 15, 2001·The Journal of Biological Chemistry·J JiangC Patterson
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·S V WesleyP M Waterhouse
May 25, 2002·The Plant Journal : for Cell and Molecular Biology·Christian S HardtkeXing Wang Deng
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jack R PeartDavid C Baulcombe
Sep 11, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Masafumi Muratani, William P Tansey
Mar 11, 2003·Nature Structural Biology·Melanie D OhiKathleen L Gould
Jun 6, 2003·Cell·Gali PragJames H Hurley
Aug 28, 2003·Microbes and Infection·Paul Muskett, Jane Parker
Dec 19, 2003·Nucleic Acids Research·Ivica LetunicPeer Bork
May 21, 2004·Nature·Steven G Thomas, Vernonica E Franklin-Tong
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Dec 17, 2004·Molecular and Cellular Biology·Emily A AndrewsFelicity Z Watts

❮ Previous
Next ❯

Citations

Jun 7, 2012·Protoplasma·Pranav Pankaj SahuManoj Prasad
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Robert B AbramovitchGregory B Martin
Mar 12, 2009·Journal of Experimental Botany·Adam CraigAri Sadanandom
Nov 10, 2007·Molecular Plant-microbe Interactions : MPMI·Saeed IrianMarilyn J Roossinck
Jan 10, 2009·Molecular Plant-microbe Interactions : MPMI·Michal R SwiderskiJonathan D G Jones
Jun 13, 2012·Plant Physiology·Daniel MarinoSusana Rivas
May 18, 2007·Annual Review of Phytopathology·Andrew F Bent, David Mackey
Mar 21, 2007·Annual Review of Phytopathology·Gerben van OoijenFrank L W Takken
Jan 9, 2014·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Manisha SharmaGirdhar K Pandey
Nov 13, 2014·Nature Communications·Kazuya IshikawaTsutomu Kawasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.