The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara

Biochemical and Biophysical Research Communications
Jian YuHe Huang

Abstract

Tara was identified as an interacting partner of guanine nucleotide exchange factor Trio and TRF1. Tara is proposed to be involved in many important fundamental cellular processes, ranging from actin remodeling, directed cell movement, to cell cycle regulation. Yet, its exact roles required further elucidation. Here, we identify a novel Tara-binding protein HECTD3, a putative member of HECT E3 ubiquitin ligases. HECTD3 directly binds Tara in vitro and forms a complex with Tara in vivo. Overexpression of HECTD3 enhances the ubiquitination of Tara in vivo and promotes the turnover of Tara, whereas depletion of HECTD3 by small interfering RNA decreases Tara degradation. Furthermore, depletion of HECTD3 leads to multipolar spindle formation. All these findings suggest that HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara.

References

Dec 8, 1995·Science·L ChongT de Lange
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J M HuibregtseP M Howley
Dec 6, 1996·Science·R W KingM W Kirschner
Feb 20, 1997·Nature·B van Steensel, T de Lange
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M HuibregtseS L Beaudenon
Apr 1, 1997·The EMBO Journal·A BianchiT de Lange
May 25, 1999·Cell·J D GriffithT de Lange
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P O'BrienM Streuli
Sep 5, 2002·Genes & Development·J Wade HarperMark J Solomon
Oct 28, 2005·Essays in Biochemistry·Sarath C DhananjayanZafar Nawaz
May 10, 2006·Biochemical and Biophysical Research Communications·Dan LiuXuebiao Yao
May 29, 2007·Biochemical and Biophysical Research Communications·Ning ZhangXuebiao Yao

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Citations

Sep 30, 2008·Cellular and Molecular Neurobiology·Lisheng ZhangNian Zhang
Jul 21, 2009·Cardiovascular Research·Pasquale FasanaroFabio Martelli
Jul 24, 2012·Experimental Cell Research·Yuanyuan ZhuHe Huang
Jan 25, 2011·Hormone Molecular Biology and Clinical Investigation·Jose RussoIrma H Russo
Oct 23, 2019·Cellular and Molecular Life Sciences : CMLS·Qiuyun JiangCeshi Chen
Jun 13, 2018·Cell Communication and Signaling : CCS·Sungjin ParkJongsun Park
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beti ZaharijaNicholas J Bradshaw
Feb 20, 2021·Pharmacology & Therapeutics·Sunil SinghJ Sivaraman

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