The E4F protein is required for mitotic progression during embryonic cell cycles

Molecular and Cellular Biology
Laurent Le CamPiotr Sicinski

Abstract

The ubiquitously expressed E4F protein was originally identified as an E1A-regulated cellular transcription factor required for adenovirus replication. The function of this protein in normal cell physiology remains largely unknown. To address this issue, we generated E4F knockout mice by gene targeting. Embryos lacking E4F die at the peri-implantation stage, while in vitro-cultured E4F(-/-) blastocysts exhibit defects in mitotic progression, chromosomal missegregation, and increased apoptosis. Consistent with these observations, we found that E4F localizes to the mitotic spindle during the M phase of early embryos. Our results establish a crucial role for E4F during early embryonic cell cycles and reveal an unexpected function for E4F in mitosis.

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Citations

Nov 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthieu LacroixLaurent Le Cam
Jun 29, 2011·The Journal of Experimental Medicine·Elodie HatchiLaurent Le Cam
Aug 3, 2006·Genes & Development·Jalila ChagraouiGuy Sauvageau
Jul 1, 2006·Molecular and Cellular Biology·Ho LeeDae-Sik Lim
Nov 15, 2012·Genes & Cancer·Ian M Love, Steven R Grossman
Jan 16, 2009·PloS One·Arikuni UchimuraTakeshi Yagi
Jan 1, 2008·Molecular and Cellular Endocrinology·Jérôme Artus, Michel Cohen-Tannoudji
Jun 9, 2007·EMBO Reports·Marija CvetanovicPuneet Opal
Dec 8, 2010·The Biochemical Journal·Jia ShenChuangui Wang
Apr 7, 2015·Cell Reports·David GroteGuy Sauvageau
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Perrine Goguet-RubioLaurent Le Cam
Sep 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Matthieu LacroixClaude Sardet
Mar 17, 2010·The Journal of Biological Chemistry·Junya NojimaTakenobu Katagiri
Sep 24, 2020·Animals : an Open Access Journal From MDPI·Rong-Ge YanQi-En Yang
Dec 7, 2018·Journal of Biochemical and Molecular Toxicology·Qiang TanLinhua Liu
Jan 8, 2020·International Journal of Molecular Sciences·Scott BangManabu Kurokawa
Mar 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Céline MoisonGuy Sauvageau
Sep 3, 2010·Journal of Cell Science·An Vanden BoschGeert Carmeliet
Sep 20, 2007·Journal of Cell Science·Howard DonningerGeoffrey J Clark
Dec 4, 2021·Nature Communications·Matthieu LacroixLaurent Le Cam

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