The Early-Acting Peroxin PEX19 Is Redundantly Encoded, Farnesylated, and Essential for Viability in Arabidopsis thaliana

PloS One
Margaret M McDonnellBonnie Bartel

Abstract

Peroxisomes are single-membrane bound organelles that are essential for normal development in plants and animals. In mammals and yeast, the peroxin (PEX) proteins PEX3 and PEX19 facilitate the early steps of peroxisome membrane protein (PMP) insertion and pre-peroxisome budding from the endoplasmic reticulum. The PEX3 membrane protein acts as a docking site for PEX19, a cytosolic chaperone for PMPs that delivers PMPs to the endoplasmic reticulum or peroxisomal membrane. PEX19 is farnesylated in yeast and mammals, and we used immunoblotting with prenylation mutants to show that PEX19 also is fully farnesylated in wild-type Arabidopsis thaliana plants. We examined insertional alleles disrupting either of the two Arabidopsis PEX19 isoforms, PEX19A or PEX19B, and detected similar levels of PEX19 protein in the pex19a-1 mutant and wild type; however, PEX19 protein was nearly undetectable in the pex19b-1 mutant. Despite the reduction in PEX19 levels in pex19b-1, both pex19a-1 and pex19b-1 single mutants lacked notable peroxisomal β-oxidation defects and displayed normal levels and localization of peroxisomal matrix and membrane proteins. The pex19a-1 pex19b-1 double mutant was embryo lethal, indicating a redundantly encoded critical ...Continue Reading

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Citations

Aug 9, 2016·Current Opinion in Plant Biology·Sigrun Reumann, Bonnie Bartel
Dec 24, 2018·Journal of Integrative Plant Biology·Ronghui PanJianping Hu
Feb 15, 2019·Journal of Integrative Plant Biology·Sarah E BurkhartBonnie Bartel

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Methods Mentioned

BETA
genotyping
electrophoresis
geranylgeranylation
confocal microscopy

Software Mentioned

SPSS
Clustal
DNAStar
MegAlign

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