PMID: 9435260Jan 22, 1998Paper

The early phase of engraftment after murine blood cell transplantation is mediated by hematopoietic stem cells

Proceedings of the National Academy of Sciences of the United States of America
J M ZijlmansW E Fibbe

Abstract

Blood cells transplantation is largely replacing bone marrow transplantation because engraftment is more rapid. This accelerated engraftment is thought to be mediated by relatively mature committed hematopoietic progenitor cells. Herein, we have used a modified rhodamine (Rho) staining procedure to identify and purify Rho+/++ (dull/bright) and Rho- (negative) subpopulations of hematopoietic progenitor cells in murine cytokine-mobilized blood. The Rho+/++ cell population contained > 99% of committed progenitor cells with in vitro colony-forming ability. The Rho- cell population contained the majority of hematopoietic stem cells with in vivo marrow repopulating ability. The rate of hematopoietic reconstitution was identical in recipients of grafts containing only purified Rho- stem cells or purified Rho- stem cells in combination with large numbers of Rho+/++ committed progenitor cells. In contrast, transplantation of 3-fold more hematopoietic stem cells resulted in accelerated reconstitution, indicating that the reconstitution rate was determined by the absolute numbers of Rho- stem cells in the graft. In addition, we observed a 5- to 8-fold reduced frequency of the subset of hematopoietic stem cells with long-term repopulating ...Continue Reading

References

Jun 1, 1992·The Journal of Experimental Medicine·C L Li, G R Johnson
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D E Harrison, R K Zhong
Feb 1, 1990·Genes & Development·C T Jordan, I R Lemischka
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J M ZijlmansW E Fibbe
Apr 1, 1993·Current Opinion in Immunology·N UchidaI L Weissman
Mar 24, 1994·The New England Journal of Medicine·J O Armitage

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Citations

Jan 22, 2004·Archives of Medical Research·Stephen J Szilvassy
Mar 29, 2001·Experimental Hematology·M PodestàA Bacigalupo
May 30, 2001·Experimental Hematology·G P NiemeyerC D Lothrop
Mar 18, 2000·Experimental Hematology·M V ZinovyevaA V Belyavsky
Feb 13, 2001·British Journal of Haematology·F de BoerG J Schuurhuis
Sep 22, 1998·The Journal of Experimental Medicine·T H BrummendorfP M Lansdorp
May 22, 2001·Journal of Hematotherapy & Stem Cell Research·L KobariL Douay
Mar 9, 2006·Stem Cells and Development·Isaac YanivNadir Askenasy
Aug 15, 1998·Vox Sanguinis·P M Lansdorp
Jun 18, 1999·Annals of the New York Academy of Sciences·D D Cooper, G J Spangrude
May 20, 2011·Journal of Virology·Ibrahim BoussaadSibylle Schneider-Schaulies
Aug 29, 2013·International Journal of Medical Sciences·Mihoko TomidaToshiyuki Kawakami
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Johannes F M PruijtWillem E Fibbe
Jul 31, 2008·Stem Cell Reviews·Peter J Quesenberry, Jason M Aliotta
Jun 11, 2008·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Yorihisa OritaNoriyuki Nagai
Jul 17, 2007·Seminars in Hematology·Robert Möhle, Lothar Kanz
Jul 6, 2005·Brain Research·Hidetsugu TsujigiwaNoriyuki Nagai
Nov 13, 2012·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Koen Van BesienAndrew Artz
Jul 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Susan R Opalenik, Jeffrey M Davidson
Jul 19, 2001·Annals of the New York Academy of Sciences·M Punzel, A D Ho

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