The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses

Proteomics
Jean-Emmanuel SarryJacques Bourguignon

Abstract

To get more insight into plant cell response to cadmium (Cd) stress, both proteomic and metabolomic "differential display" analyses were performed on Arabidopsis thaliana cells exposed to different concentrations of the toxic chemical. After a 24 h treatment, soluble proteins extracted from untreated and treated cells were separated by 2-D-PAGE and image analyses were performed to quantify and compare protein levels. Proteins up- and down-regulated in response to Cd were identified by MS and mapped into specific metabolic pathways and cellular processes, highlighting probable activation of the carbon, nitrogen, and sulfur metabolic pathways. For some of these proteins, Northern blot and RT-PCR analyses were performed to test transcript accumulation in response to Cd. In parallel, metabolite profiling analyses by LC coupled to ESI MS were initiated to better characterize the metabolic adaptation to the chemical stress. This study revealed that the main variation at the metabolite level came from the presence of six different families of phytochelatins, in A. thaliana cells treated with Cd, whose accumulation increases with Cd concentrations. Taken together these data provide an overview of the molecular and cellular changes elic...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·K Biemann
Jan 1, 1990·Annual Review of Biochemistry·W E Rauser
Mar 1, 1995·The Plant Journal : for Cell and Molecular Biology·P MeuwlyW E Rauser
Apr 1, 1995·Plant Physiology·R HowdenC S Cobbett
Mar 3, 1995·The Journal of Biological Chemistry·D F OrtizD W Ow
Sep 1, 1993·Plant Physiology·M AxelosB Lescure
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·O K VatamaniukP A Rea
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J M MouillonF Rébeillé
Feb 19, 2000·Phytochemistry·H KubotaT Maitani
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·N S PenceL V Kochian
Jul 13, 2000·Plant Physiology·C S Cobbett
Nov 18, 2000·The Journal of Biological Chemistry·K VidoJ Labarre
Apr 5, 2001·Trends in Plant Science·R DouceF Rébeillé
Jul 2, 2002·Plant Molecular Biology·Ulrich WagnerFelix Mauch
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Feb 1, 1995·Plant Physiology·S. KlapheckL. Bergmann
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Laetitia Perfus-BarbeochCyrille Forestier
May 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicolas BouchéHillel Fromm
May 22, 2003·Journal of Plant Physiology·Houda GouiaMohamed Habib Ghorbal
Jun 14, 2003·Plant, Cell & Environment·I. FinkemeierK.-J. Dietz
Sep 25, 2003·Annual Review of Plant Biology·Karl-Josef Dietz
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Miguel A Rodriguez MillaJ Perry Gustafson
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Philip A. ReaEnrico Martinoia
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Kathleen A. Marrs
Jan 1, 1997·Environmental Pollution·P DasG R Rout
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masami Yokota HiraiKazuki Saito
Jun 23, 2004·Journal of Experimental Botany·Stanislav Kopriva, Anna Koprivova
Jun 23, 2004·Journal of Experimental Botany·Masami Yokota Hirai, Kazuki Saito

❮ Previous
Next ❯

Citations

Jul 11, 2009·Analytical and Bioanalytical Chemistry·Anja BräutigamDirk Wesenberg
Jul 16, 2010·Analytical and Bioanalytical Chemistry·Anja BräutigamDirk Schaumlöffel
Jul 12, 2011·Planta·Latifa Boulila-ZoghlamiRenaud Brouquisse
Aug 10, 2010·Plant Molecular Biology·Zhifang WangFangsen Xu
Nov 15, 2006·Photosynthesis Research·Sylvie GilletPierre Le Maréchal
Dec 11, 2008·Environmental Science and Pollution Research International·Abdul R Memon, Peter Schröder
Jul 7, 2012·Environmental Science and Pollution Research International·Xue-Ping LingHe-Qing Huang
Jul 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Peter StrebRichard Bligny
Apr 16, 2011·Environmental Science & Technology·Marta MarmiroliNelson Marmiroli
Dec 2, 2010·Journal of Proteome Research·Chika C Nwugo, Alfredo J Huerta
Jan 6, 2010·Journal of Experimental Botany·Marjo TuomainenSirpa Kärenlampi
Dec 2, 2011·Journal of Experimental Botany·Florent VilliersVéronique Hugouvieux
Sep 1, 2012·Journal of Experimental Botany·Yin An YaoGang Xu
Dec 21, 2012·Journal of Experimental Botany·Jonas Van BockhavenMonica Höfte
Apr 24, 2012·Tree Physiology·Brahma Reddy InduriStephen P DiFazio
Nov 11, 2011·International Journal of Molecular Sciences·Els KeunenAnn Cuypers
Feb 20, 2014·Metallomics : Integrated Biometal Science·Fany DoustalyJacques Bourguignon
Jun 29, 2014·Journal of Plant Physiology·Candida VanniniMarcella Bracale
Jul 1, 2014·Metallomics : Integrated Biometal Science·Siavash ForoughiDamien L Callahan
Jul 16, 2014·Plant Physiology and Biochemistry : PPB·Rosalía Cristina PazOscar Adolfo Ruíz
Jul 30, 2014·Archives of Environmental Contamination and Toxicology·Eugeniusz ParysElżbieta Romanowska
Sep 1, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Lupeng XieFeibo Wu
Apr 7, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ganesh ThapaLingaraj Sahoo
Aug 21, 2012·Cellular and Molecular Life Sciences : CMLS·Ya-Fen Lin, Mark G M Aarts
Sep 17, 2011·Metallomics : Integrated Biometal Science·Sean C BoothRaymond J Turner
Jun 2, 2012·Journal of Proteomics·Zahed HossainSetsuko Komatsu
Dec 16, 2011·Journal of Plant Physiology·Elżbieta RomanowskaMaksymilian Zienkiewicz
Sep 11, 2010·Journal of Plant Physiology·Ann CuypersJaco Vangronsveld

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.