The Effect of 0.5% Timolol Maleate on Corneal(Lymph)Angiogenesis in a Murine Suture Model

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
Yang Kyung ChoBalamurali K Ambati

Abstract

To investigate the anti(lymph)angiogenic and anti-inflammatory effects of 0.5% timolol maleate in a murine corneal suture model. Corneal neovascularization and lymphangiogenesis were compared in groups of mice that underwent corneal suture and were subsequently administered a subconjunctival injection of 0.5% timolol maleate, dexamethasone, or phosphate-buffered saline (PBS). Immunohistochemical staining and analysis were performed in each group. Real-time polymerase chain reaction (RT-PCR) was performed to quantify the expression of inflammatory cytokines [TNF-alpha and interleukin (IL)-6], vascular endothelial growth factor (VEGF)-A, VEGF-C, vascular endothelial growth factor receptor (VEGFR)-2, and VEGFR-3. When corneas from the timolol-treated group were compared to the PBS-treated group, we observed decreases in angiogenesis, lymphangiogenesis, and inflammatory infiltration in the timolol-treated group (P value <0.05 in all respective comparisons). Corneas from the timolol-treated group showed reduced expression of VEGF-A, VEGF-C, TNF-alpha, IL-6, VEGFR-2, and VEGFR-3 compared to corneas from the PBS group (P value <0.05 in all respective comparisons). Blocking adrenergic signaling in the cornea with 0.5% timolol maleate d...Continue Reading

References

Oct 20, 2006·Nature·Balamurali K AmbatiJayakrishna Ambati
Jan 4, 2008·Investigative Ophthalmology & Visual Science·Shulamit SchwartzAdiel Barak
Apr 28, 2012·European Journal of Immunology·Roel G J Klein WolterinkRudi W Hendriks
Sep 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hironori UeharaBalamurali K Ambati
Dec 2, 2015·Experimental and Therapeutic Medicine·Shanying WuZugen Huang
Jan 1, 2016·Investigative Ophthalmology & Visual Science·Ramin NouriniaNader Sheibani
Feb 4, 2016·World Journal of Cardiology·Israel Fernandez-PinedaRyan Jones

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Citations

Apr 30, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Yang Kyung ChoBalamurali Ambati
Sep 13, 2018·Cardiovascular & Hematological Disorders Drug Targets·Tahereh FarkhondehAli M P Shahri
Nov 9, 2018·Medicine·Luca FilippiElena Piozzi

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

SPSS
Image J

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