The effect of 12-myristate 13-acetate phorbol ester on human sperm hyperactivation

Fertility and Sterility
Z Zhang, H W Baker

Abstract

To determine whether 12-myristate 13-acetate phorbol ester (PMA) can increase hyperactivated motility of human sperm. A controlled pharmacological study using computer-assisted semen analysis. Andrology laboratory in a medical research institution. Normal semen was obtained from 48 men. Washed sperm were exposed to different concentrations of PMA alone or with P and pentoxifylline (PTX) for up to 2 hours and sperm motility measured by a computer-assisted semen analyzer. The percentage of sperm with hyperactivated motility was determined from the motility parameters: curvilinear velocity, linearity, and maximum amplitude of lateral head displacement. Phorbol ester PMA increased hyperactivated motility in a dose- and time-dependent manner. At 1 hour, the average increases in hyperactivated motility were as follows: 2 microM, 4.8% +/- 1.5%; 6 microM, 9.6% +/- 1.5%; and 20 microM, 11.3% +/- 2.2%. The PMA effect was not altered when P or PTX were added although each separately had a positive effect on hyperactivation. Phorbol ester PMA stimulates human sperm hyperactivated motility, indicating the involvement of protein kinase C in the signal transduction pathway.

References

Oct 1, 1970·Journal of Reproduction and Fertility·R Yanagimachi
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B K DavisK Bedigian
Jun 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·E BaldiG Forti
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J YangP Jouannet

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Citations

Jul 4, 2002·Fertility and Sterility·De Yi LiuH W Gordon Baker
Jun 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Longmei ZhaoDavid J Miller

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