PMID: 9005659Mar 1, 1996Paper

The effect of 2,4-dinitrophenol and glucose on the utilization of succinate, lactate, pyruvate and formate by Escherichia coli K12

Ukrainskiĭ biokhimicheskiĭ zhurnal
M V Dyl'ovyĭ

Abstract

The effect of protonophore 2,4-dinitrophenol (5-25 microM) in lithium chloride acidic medium (pH 4.5) on utilization of acidic energetic substrates was studied in the presence and absence of glucose. The rate of succinate, lactate and pyruvate utilization by AB 1157 strains was two times lower, than by the KS 400 strain. Isomolar glucose concentration (0.5 mM) decreased the utilization rate of succinate by 60% pyruvate by 30% the AB 1157 strain being much more sensitive than the KS 400 strain both to the action of glucose and to the reaction to glucose and 2,4-dinitrophenol. Acidification of the medium by bacteria has been observed, which shows changes in energy metabolism (glycolysis takes place instead of oxidation of substrates) under conditions of intensive aeration. The results obtain permit assuming that the AB 1157 strain has a certain defect of the membrane which causes increased proton conduction.

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