The effect of 5,8,11,14-eicosatetraynoic acid on endothelial cell gene expression

European Journal of Pharmacology
K M StuhlmeierF H Bach

Abstract

The endothelium plays a key role in inflammation, hemostasis and organ rejection. We report here that a synthetic polyunsaturated fatty acid, 5,8,11,14-eicosatetraynoic acid (ETYA), selectively inhibits the up-regulation of several genes on endothelial cells. ETYA suppresses endothelial cell activation by inhibiting the up-regulation of adhesion molecules like E-selectin. A runoff assay for E-selectin demonstrated that the suppression is at the level of transcription. The fact that ETYA inhibits E-selectin upon stimulation with a diverse group of stimuli like lipopolysaccharide, tumor necrosis factor-alpha or phorbol 12-myristate 13-acetate, suggests that ETYA does not exert its effect by modifying membrane-bound receptors. The messenger RNA for interleukin-8 and glyceraldehyde phosphate dehydrogenase are not affected. Pre-treatment of endothelial cells with ETYA also prevents the adherence of monocytes to tumor necrosis factor-alpha-stimulated cells.

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Citations

Jan 16, 1999·Biochemical Pharmacology·K M StuhlmeierJ J Kao
Aug 6, 1999·Biochimica Et Biophysica Acta·K M Stuhlmeier, Y Lin
Feb 15, 2000·European Journal of Biochemistry·K M Stuhlmeier
May 18, 2005·Microvascular Research·Natalia V BogatchevaAlexander D Verin

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