PMID: 7545282May 1, 1995Paper

The effect of a chemical phosphatase on single calcium channels and the inactivation of whole-cell calcium current from isolated guinea-pig ventricular myocytes

Pflügers Archiv : European journal of physiology
T J Allen, R A Chapman

Abstract

A chemical phosphatase, butanedione monoxime (BDM, at 12-20 mM), reduced open probability (P0) of single cardiac L-type Ca2+ channels in cell-attached patches from guinea-pig ventricular myocytes, without effect on the amplitude of single-channel current, the mean open time or the mean shorter closed time, but it increased mean longer closed time and caused a fall in channel availability. A decrease in the mean time between first channel opening and last closing within a trace was principally due to an inhibition of the longer periods of activity. As a result, the time course of the mean currents, which resolved into an exponentially declining and a sustained component, was changed by an increase in the rate of the exponential phase and a profound reduction of the sustained current. Essentially similar results were obtained when studying whole-cell Ba2+ currents. The inactivation of the whole-cell Ca2+ currents was composed of two exponentially declining components with the slower showing a significantly greater sensitivity to BDM, an effect that was much more pronounced in myocytes exposed to isoprenaline with adenosine 5'-O-(3-thiotriphosphate) (ATP[gamma S]) in the pipette solution. The actions of BDM, which are the opposite...Continue Reading

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Citations

Oct 1, 1996·Naunyn-Schmiedeberg's Archives of Pharmacology·N ZimmermannH Scholz
May 1, 1996·British Journal of Pharmacology·T J Allen, R A Chapman
Jul 1, 1997·Journal of Cardiovascular Electrophysiology·Y ChengT N Mazgalev
Jun 6, 1998·The Journal of Physiology·T J AllenA C Dolphin
Nov 18, 2003·The Journal of Physiology·Min WangJoshua R Berlin
Jun 16, 2009·Regulatory Peptides·Jing Yu JinKyung Woo Cho
Jun 11, 2002·Biochemical and Biophysical Research Communications·E A LukyanetzP G Kostyuk
Mar 5, 1999·Clinical and Experimental Pharmacology & Physiology·P C DooleyR A Chapman
Apr 3, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Amadou K S CamaraDavid F Stowe
Sep 4, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jianqing ZhangMary O Gray
Aug 16, 2000·American Journal of Physiology. Cell Physiology·S MitaraiK Taniyama

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